『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=309866EID:309866, Map:0, LastModified:2017年8月27日(日) 21:27:54, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[船木 真理], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Inukai K (日) (読)
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学籍番号 (推奨):
[継承]
2. (英) Asano T (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Katagiri H (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Anai M (日) (読)
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学籍番号 (推奨):
[継承]
5.船木 真理 ([徳島大学.病院.中央診療施設等.糖尿病対策センター])
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学籍番号 (推奨):
[継承]
6. (英) Ishihara H (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Tsukuda K (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Kikuchi M (日) (読)
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学籍番号 (推奨):
[継承]
9. (英) Yazaki Y (日) (読)
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学籍番号 (推奨):
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10. (英) Oka Y (日) (読)
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[継承]
題名 (必須): (英) Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter.  (日)    [継承]
副題 (任意):
要約 (任意): (英) A mutated GLUT1 glucose transporter, a Trp-388, 412 mutant whose tryptophans 388 and 412 were both replaced by leucines, was constructed by site-directed mutagenesis and expressed in Chinese hamster ovary cells. Glucose transport activity was decreased to approx. 30% in the Trp-388, 412 mutant compared with that in the wild type, a similar decrease in transport activity had been observed previously in the Trp-388 mutant and the Trp-412 mutant which had leucine at 388 and 412 respectively. Cytochalasin B labelling of the Trp-388 mutant was only decreased rather than abolished, a result similar to that obtained previously for the Trp-412 mutant. Cytochalasin B labelling was finally abolished completely in the Trp-388, 412 mutant, while cytochalasin B binding to this mutant was decreased to approx. 30% of that of the wild-type GLUT1 at the concentration used for photolabelling. This level of binding is thought to be adequate to detect labelling, assuming that the labelling efficiency of these transporters is similar. These findings suggest that cytochalasin B binds to the transmembrane domain of the glucose transporter in the vicinity of helix 10-11, and is inserted covalently by photoactivation at either the 388 or the 412 site.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Binding Sites (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) CHO Cells (日) (読) [継承]
5. (英) Cricetinae (日) (読) [継承]
6. (英) Cytochalasin B (日) (読) [継承]
7. (英) Deoxyglucose (日) (読) [継承]
8. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
9.グルコース (glucose) [継承]
10. (英) Glucose Transporter Type 1 (日) (読) [継承]
11. (英) Leucine (日) (読) [継承]
12. (英) Monosaccharide Transport Proteins (日) (読) [継承]
13. (英) Mutagenesis, Site-Directed (日) (読) [継承]
14. (英) Mutation (日) (読) [継承]
15. (英) Tryptophan (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 302 ( Pt 2) [継承]
(必須):
(必須): 355 361 [継承]
都市 (任意):
年月日 (必須): 西暦 1994年 9月 1日 (平成 6年 9月 1日) [継承]
URL (任意):
DOI (任意):
PMID (任意): 8092986    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● K Inukai, T Asano, H Katagiri, M Anai, Makoto Funaki, H Ishihara, K Tsukuda, M Kikuchi, Y Yazaki and Y Oka : Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter., The Biochemical Journal, Vol.302 ( Pt 2), (号), 355-361, 1994.
欧文冊子 ● K Inukai, T Asano, H Katagiri, M Anai, Makoto Funaki, H Ishihara, K Tsukuda, M Kikuchi, Y Yazaki and Y Oka : Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter., The Biochemical Journal, Vol.302 ( Pt 2), (号), 355-361, 1994.

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