| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Ogihara T (日) (読)
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| 2. | (英) Isobe T (日) (読)
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| 3. | (英) Ichimura T (日) (読)
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| 4. | (英) Taoka M (日) (読)
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| 5. | 船木 真理
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| 6. | (英) Sakoda H (日) (読)
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| 7. | (英) Onishi Y (日) (読)
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| 8. | (英) Inukai K (日) (読)
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| 9. | (英) Anai M (日) (読)
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| 10. | (英) Fukushima Y (日) (読)
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| 11. | (英) Kikuchi M (日) (読)
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| 12. | (英) Yazaki Y (日) (読)
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| 13. | (英) Oka Y (日) (読)
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| 14. | (英) Asano T (日) (読)
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| ○題名 (必須): | □ | (英) 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Insulin binding to its receptor induces the phosphorylation of cytosolic substrates, insulin receptor substrate (IRS)-1 and IRS-2, which associate with several Src homology-2 domain-containing proteins. To identify unique IRS-1-binding proteins, we screened a human heart cDNA library with 32P-labeled recombinant IRS-1 and obtained two isoforms (epsilon and zeta) of the 14-3-3 protein family. 14-3-3 protein has been shown to associate with IRS-1 in L6 myotubes, HepG2 hepatoma cells, Chinese hamster ovary cells, and bovine brain tissue. IRS-2, a protein structurally similar to IRS-1, was also shown to form a complex with 14-3-3 protein using a baculovirus expression system. The amount of 14-3-3 protein associated with IRS-1 was not affected by insulin stimulation but was increased significantly by treatment with okadaic acid, a potent serine/threonine phosphatase inhibitor. Peptide inhibition experiments using phosphoserine-containing peptides of IRS-1 revealed that IRS-1 contains three putative binding sites for 14-3-3 protein (Ser-270, Ser-374, and Ser-641). Among these three, the motif around Ser-270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. Indeed, a truncated mutant of IRS-1 consisting of only the phosphotyrosine binding domain retained the capacity to bind to 14-3-3 protein in vivo. Finally, the effect of 14-3-3 protein binding on the insulin-induced phosphorylation of IRS-1 was investigated. Phosphoamino acid analysis revealed that IRS-1 coimmunoprecipitated with anti-14-3-3 antibody to be weakly phosphorylated after insulin stimulation, on tyrosine as well as serine residues, compared with IRS-1 immunoprecipitated with anti-IRS-1 antibody. Thus, the association with 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. (日)
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| ○キーワード (推奨): | 1. | (英) 14-3-3 Proteins (日) (読)
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| 2. | アデノシン三リン酸 (adenosine triphosphate)
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| 3. | (英) Amino Acid Sequence (日) (読)
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| 4. | (英) Animals (日) (読)
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| 5. | (英) Binding Sites (日) (読)
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| 6. | 脳 (brain)
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| 7. | (英) CHO Cells (日) (読)
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| 8. | (英) Carcinoma, Hepatocellular (日) (読)
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| 9. | (英) Cattle (日) (読)
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| 10. | (英) Cell Line (日) (読)
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| 11. | (英) Cricetinae (日) (読)
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| 12. | (英) Gene Library (日) (読)
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| 13. | (英) Humans (日) (読)
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| 14. | インスリン (insulin)
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| 15. | (英) Insulin Receptor Substrate Proteins (日) (読)
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| 16. | (英) Intracellular Signaling Peptides and Proteins (日) (読)
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| 17. | (英) Liver Neoplasms (日) (読)
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| 18. | (英) Mice (日) (読)
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| 19. | (英) Molecular Sequence Data (日) (読)
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| 20. | 心筋 (myocardium)
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| 21. | (英) Okadaic Acid (日) (読)
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| 22. | (英) Phosphoproteins (日) (読)
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| 23. | (英) Phosphorus Radioisotopes (日) (読)
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| 24. | (英) Phosphotyrosine (日) (読)
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| 25. | (英) Protein Biosynthesis (日) (読)
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| 26. | (英) Proteins (日) (読)
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| 27. | (英) Rats (日) (読)
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| 28. | (英) Receptor, Insulin (日) (読)
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| 29. | (英) Recombinant Proteins (日) (読)
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| 30. | (英) Sequence Alignment (日) (読)
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| 31. | (英) Sequence Homology, Amino Acid (日) (読)
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| 32. | (英) Spodoptera (日) (読)
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| 33. | (英) Transfection (日) (読)
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| 34. | (英) Tumor Cells, Cultured (日) (読)
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| 35. | (英) Tyrosine 3-Monooxygenase (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
| ○ISSN (任意): | □ | 0021-9258
ISSN: 0021-9258
(pISSN: 0021-9258, eISSN: 1083-351X) Title: The Journal of biological chemistryTitle(ISO): J Biol ChemPublisher: American Society for Biochemistry and Molecular Biology (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 272
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| ○号 (必須): | □ | 40
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| ○頁 (必須): | □ | 25267 25274
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 1997年 10月 3日 (平成 9年 10月 3日)
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| ○URL (任意): |
| ○DOI (任意): |
| ○PMID (任意): | □ | 9312143 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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