『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=309343)

EID=309343EID:309343, Map:0, LastModified:2018年3月7日(水) 16:37:48, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[[センター長]/[徳島大学.農工商連携センター]], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.農工商連携センター (2012年4月1日〜2016年3月31日) [継承]
著者 (必須): 1. (英) Takashi Kawase (日) 川瀬 貴士 (読) かわせ たかし
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.菅野 茂夫
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Tomoo Shimada (日) 嶋田 知生 (読) しまだ ともお
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Ikuko Hara-Nishimura (日) 西村 いくこ (読) にしむら いくこ
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) A direction-selective local-thresholding method, DSLT, in combination with a dye-based method for automated three-dimensional segmentation of cells and airspaces in developing leaves.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Quantifying the anatomical data acquired from three-dimensional (3D) images has become increasingly important in recent years. Visualization and image segmentation are essential for acquiring accurate and detailed anatomical data from images; however, plant tissues such as leaves are difficult to image by confocal or multi-photon laser scanning microscopy because their airspaces generate optical aberrations. To overcome this problem, we established a staining method based on Nile Red in silicone-oil solution. Our staining method enables color differentiation between lipid bilayer membranes and airspaces, while minimizing any damage to leaf development. By repeated applications of our staining method we performed time-lapse imaging of a leaf over 5 days. To counteract the drastic decline in signal-to-noise ratio at greater tissue depths, we also developed a local thresholding method (direction-selective local thresholding, DSLT) and an automated iterative segmentation algorithm. The segmentation algorithm uses the DSLT to extract the anatomical structures. Using the proposed methods, we accurately segmented 3D images of intact leaves to single-cell resolution, and measured the airspace volumes in intact leaves.  (日)    [継承]
キーワード (推奨): 1. (英) Algorithms (日) (読) [継承]
2. (英) Arabidopsis (日) (読) [継承]
3. (英) Imaging, Three-Dimensional (日) (読) [継承]
4. (英) Microscopy, Confocal (日) (読) [継承]
5. (英) Oxazines (日) (読) [継承]
6. (英) Plant Leaves (日) (読) [継承]
7. (英) Silicone Oils (日) (読) [継承]
8. (英) Tobacco (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Plant Journal : for Cell and Molecular Biology (Society for Experimental Biology (Great Britain))
(pISSN: 0960-7412, eISSN: 1365-313X)

ISSN (任意): 1365-313X
ISSN: 0960-7412 (pISSN: 0960-7412, eISSN: 1365-313X)
Title: The Plant journal : for cell and molecular biology
Title(ISO): Plant J
Supplier: Society for Experimental Biology
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 81 [継承]
(必須): 2 [継承]
(必須): 357 366 [継承]
都市 (任意):
年月日 (必須): 西暦 2015年 1月 初日 (平成 27年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/tpj.12738    (→Scopusで検索) [継承]
PMID (任意): 25440085    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意): 000347830900015 [継承]
Scopus (任意): 2-s2.0-84920654571 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1111/tpj.12738  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) KeywordList.Keyword: Arabidopsis thaliana  (日)    [継承]
4.(英) KeywordList.Keyword: Nicotiana glauca  (日)    [継承]
5.(英) KeywordList.Keyword: Nile Red  (日)    [継承]
6.(英) KeywordList.Keyword: Spirodela polyrhiza  (日)    [継承]
7.(英) KeywordList.Keyword: automated segmentation  (日)    [継承]
8.(英) KeywordList.Keyword: confocal microscopy  (日)    [継承]
9.(英) KeywordList.Keyword: local thresholding  (日)    [継承]
10.(英) KeywordList.Keyword: silicone oil  (日)    [継承]
11.(英) KeywordList.Keyword: technical advance  (日)    [継承]

標準的な表示

和文冊子 ● Kawase Takashi, Sigeo Sugano, Shimada Tomoo and Hara-Nishimura Ikuko : A direction-selective local-thresholding method, DSLT, in combination with a dye-based method for automated three-dimensional segmentation of cells and airspaces in developing leaves., The Plant Journal : for Cell and Molecular Biology, Vol.81, No.2, 357-366, 2015.
欧文冊子 ● Kawase Takashi, Sigeo Sugano, Shimada Tomoo and Hara-Nishimura Ikuko : A direction-selective local-thresholding method, DSLT, in combination with a dye-based method for automated three-dimensional segmentation of cells and airspaces in developing leaves., The Plant Journal : for Cell and Molecular Biology, Vol.81, No.2, 357-366, 2015.

関連情報

Number of session users = 1, LA = 0.43, Max(EID) = 405267, Max(EOID) = 1099220.