『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=308420EID:308420, Map:0, LastModified:2016年11月24日(木) 17:04:41, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[近藤 和也], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨): 学内共著 (徳島大学内研究者との共同研究 (学外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kondo Hiroshi (日) (読)
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2.三好 圭子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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[継承]
3.先山 正二
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[継承]
4.丹黒 章 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.胸部・内分泌・腫瘍外科学])
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[継承]
5.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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[継承]
題名 (必須): (英) Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones  (日)    [継承]
副題 (任意):
要約 (任意): (英) Stem cell therapy appears to be promising for restoring damaged or irreparable lung tissue. However, establishing a simple and reproducible protocol for preparing lung progenitor populations is difficult because the molecular basis for alveolar epithelial cell differentiation is not fully understood. We investigated an in vitro system to analyze the regulatory mechanisms of alveolus-specific gene expression using a human alveolar epithelial type II (ATII) cell line, A549. After cloning A549 subpopulations, each clone was classified into five groups according to cell morphology and marker gene expression. Two clones (B7 and H12) were further analyzed. Under serum-free culture conditions, surfactant protein C (SPC), an ATII marker, was upregulated in both H12 and B7. Aquaporin 5 (AQP5), an ATI marker, was upregulated in H12 and significantly induced in B7. When the RAS/MAPK pathway was inhibited, SPC and thyroid transcription factor-1 (TTF-1) expression levels were enhanced. After treatment with dexamethasone (DEX), 8-bromoadenosine 3'5'-cyclic monophosphate (8-Br-cAMP), 3-isobutyl-1-methylxanthine (IBMX), and keratinocyte growth factor (KGF), surfactant protein B and TTF-1 expression levels were enhanced. We found that A549-derived clones have plasticity in gene expression of alveolar epithelial differentiation markers and could be useful in studying ATII maintenance and differentiation.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Stem Cells International (Hindawi Publishing Corporation)
(pISSN: 1687-966X, eISSN: 1687-9678)

ISSN (任意): 1687-966X
ISSN: 1687-966X (pISSN: 1687-966X, eISSN: 1687-9678)
Title: Stem cells international
Title(ISO): Stem Cells Int
Publisher: Hindawi
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 2015 [継承]
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(必須): 165867 165867 [継承]
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年月日 (必須): 西暦 2015年 6月 17日 (平成 27年 6月 17日) [継承]
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DOI (任意): 10.1155/2015/165867    (→Scopusで検索) [継承]
PMID (任意): 26167183    (→Scopusで検索) [継承]
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WOS (任意): 000357212200001 [継承]
Scopus (任意): 2-s2.0-84934273618 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1155/2015/165867  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) OtherID: PMC4488158  (日)    [継承]

標準的な表示

和文冊子 ● Hiroshi Kondo, Keiko Miyoshi, Shoji Sakiyama, Akira Tangoku and Takafumi Noma : Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones, Stem Cells International, Vol.2015, 165867, 2015.
欧文冊子 ● Hiroshi Kondo, Keiko Miyoshi, Shoji Sakiyama, Akira Tangoku and Takafumi Noma : Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones, Stem Cells International, Vol.2015, 165867, 2015.

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