『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須):
言語 (必須): 英語 [継承]
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組織 (推奨):
著者 (必須): 1. (英) García-Rocha M (日) (読)
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2. (英) Roca A (日) (読)
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3. (英) De La Iglesia N (日) (読)
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4.馬場 麻人 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔顎顔面形態学])
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5. (英) Fernández-Novell J M (日) (読)
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6. (英) Ferrer J C (日) (読)
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7. (英) Guinovart J J (日) (読)
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題名 (必須): (英) Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes.  (日)    [継承]
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要約 (任意): (英) Changes in the intracellular distribution of liver glycogen synthase (GS) might constitute a new regulatory mechanism for the activity of this enzyme at cellular level. Our previous studies indicated that incubation of isolated hepatocytes with glucose activated GS and resulted in its translocation from a homogeneous cytosolic distribution to the cell periphery. These studies also suggested a relationship with insoluble elements of the cytoskeleton, in particular actin. Here we show the translocation of GS in a different experimental model that allows the analysis of this phenomenon in long-term studies. We describe the reversibility of translocation of GS and its effect on glycogen distribution. Incubation of cultured rat hepatocytes with glucose activated GS and triggered its translocation to the hepatocyte periphery. The relative amount of the enzyme concentrated near the plasma membrane increased with time up to 8 h of incubation with glucose, when the glycogen stores reached their maximal value. The lithium-induced covalent activation of GS was not sufficient to cause its translocation to the cell periphery. The intracellular distribution of GS closely resembled that of glycogen. Our results showed an interaction between GS and an insoluble element of the hepatocyte matrix. Although no co-localization between actin filaments and GS was observed in any condition, disruption of actin cytoskeleton resulted in a significantly lower percentage of cells in which the enzyme translocated to the cell periphery in response to glucose. This observation suggests that the microfilament network has a role in the translocation of GS.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cell Membrane (日) (読) [継承]
3. (英) Cells, Cultured (日) (読) [継承]
4. (英) Cytosol (日) (読) [継承]
5.グルコース (glucose) [継承]
6. (英) Glycogen Synthase (日) (読) [継承]
7. (英) Hepatocytes (日) (読) [継承]
8. (英) Kinetics (日) (読) [継承]
9. (英) Liver Glycogen (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Protein Transport (日) (読) [継承]
12. (英) Rats (日) (読) [継承]
13. (英) Rats, Wistar (日) (読) [継承]
14. (英) Subcellular Fractions (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Biochemical Journal ([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)

ISSN (任意): 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 357 [継承]
(必須): Pt 1 [継承]
(必須): 17 24 [継承]
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年月日 (必須): 西暦 2001年 7月 1日 (平成 13年 7月 1日) [継承]
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PMID (任意): 11415431    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : M García-Rocha, A Roca, N La Iglesia De, Otto Baba, M J Fernández-Novell, C J Ferrer and J J Guinovart : Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes., The Biochemical Journal, Vol.357, No.Pt 1, 17-24, (発行所), (都市), July 2001.
欧文冊子 ● (種別) : M García-Rocha, A Roca, N La Iglesia De, Otto Baba, M J Fernández-Novell, C J Ferrer and J J Guinovart : Intracellular distribution of glycogen synthase and glycogen in primary cultured rat hepatocytes., The Biochemical Journal, Vol.357, No.Pt 1, 17-24, (発行所), (都市), July 2001.

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