| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
| [継承] |
| ○カテゴリ (推奨): | □ | 研究
| [継承] |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.先端技術科学教育部.物質生命システム工学専攻.生命テクノサイエンスコース (2012年4月1日〜)
| [継承] |
| ○著者 (必須): | 1. | 田端 厚之 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生命科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | (英) Yukihisa Ohkubo (日) 大久保 行将 (読) おおくぼ ゆきひさ
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 3. | (英) Anyhoji Natsuki (日) 安養寺 夏希 (読) あんようじ なつき
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | (英) Hojo Keiko (日) 北條 恵子 (読) ほうじょう けいこ
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | 友安 俊文 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | (英) Tatematsu Youhei (日) 立松 洋平 (読) たてまつ ようへい
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 7. | 大倉 一人 ([名古屋大学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 8. | 長宗 秀明
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Development of a Sortase A-mediated Peptide-labeled Liposome Applicable to Drug-delivery Systems (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) In order to develop an efficient drug-delivery system (DDS), a lipopeptide-loaded liposome that functions as a platform for the transpeptidase reaction mediated by sortase A (SrtA) was constructed and its stability, as well as cell-specific targeting were evaluated in the present study. Several lipopeptides possessing an acceptor peptide sequence (oligoglycine ≥ three residues) or donor peptide sequence (LPETG) for the SrtA-mediated reaction were chemically synthesized and then inserted into the liposome membrane composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (DPPC-Chol-lipo) to obtain the lipopeptide-loaded liposomes. The transpeptidase reaction mediated by recombinant SrtA (His-ΔN59SrtA) was employed to modify the peptide moiety on the liposomal surface using a fluorescently-labeled substrate peptide corresponding to the species of each loaded lipopeptide. Furthermore, lung tumor-binding peptide (LTBP)-labeled liposomes, prepared by this transpeptidase reaction, were investigated for selective targeting to lung cancer cells in vitro. The His-ΔN59SrtA-mediated transpeptidation of fluorescently-labeled peptide on the lipopeptide-loaded DPPC-Chol-lipo was confirmed. The selective targeting of LTBP-labeled liposomes to the lung cancer cell line A549 was also observed in vitro. These results suggest that the labeling of acceptor or donor lipopeptide-loaded liposomes with the transpeptidase SrtA could be a useful method for developing a platform applicable to a cancer-targeting DDS. (日)
| [継承] |
| ○キーワード (推奨): | 1. | ドラッグデリバリーシステム (drug delivery system/->キーワード[薬物送達システム])
| [継承] |
| 2. | リポソーム (liposomes)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Anticancer Research (International Institute of Anticancer Research(IIAR))
(pISSN: 0250-7005, eISSN: 1791-7530)
| ○ISSN (任意): | □ | 1791-7530
ISSN: 0250-7005
(pISSN: 0250-7005, eISSN: 1791-7530) Title: Anticancer researchTitle(ISO): Anticancer ResPublisher: International Institute of Anticancer Research (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 35
| [継承] |
| ○号 (必須): | □ | 8
| [継承] |
| ○頁 (必須): | □ | 4411 4417
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2015年 8月 初日 (平成 27年 8月 初日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): |
| ○PMID (任意): | □ | 26168480 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): | □ | 000359294900006
| [継承] |
| ○Scopus (任意): | □ | 2-s2.0-84939824857
| [継承] |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
| [継承] |
| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
| [継承] |
| 3. | (英) KeywordList.Keyword: Drug-delivery system (日)
| [継承] |
| 4. | (英) KeywordList.Keyword: liposome (日)
| [継承] |
| 5. | (英) KeywordList.Keyword: sortase A (日)
| [継承] |