『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=306646)

EID=306646EID:306646, Map:0, LastModified:2016年5月24日(火) 18:02:07, Operator:[岡村 永一], Avail:TRUE, Censor:0, Owner:[岡村 永一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) HItomi Matsuzaki (日) (読)
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[継承]
2.岡村 永一
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学籍番号 (推奨):
[継承]
3. (英) Takuya Takahashi (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Aki Ushiki (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Toshinobu Nakamura (日) (読)
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学籍番号 (推奨):
[継承]
6. (英) Toru Nakano (日) (読)
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[継承]
7. (英) Kenichiro Hata (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Akiyoshi Fukamizu (日) (読)
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[継承]
9. (英) Keiji Tanimoto (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Genomic imprinting is a major monoallelic gene expression regulatory mechanism in mammals, and depends on gamete-specific DNA methylation of specialized cis-regulatory elements called imprinting control regions (ICRs). Allele-specific DNA methylation of the ICRs is faithfully maintained at the imprinted loci throughout development, even in early embryos where genomes undergo extensive epigenetic reprogramming, including DNA demethylation, to acquire totipotency. We previously found that an ectopically introduced H19 ICR fragment in transgenic mice acquired paternal allele-specific methylation in the somatic cells of offspring, whereas it was not methylated in sperm, suggesting that its gametic and postfertilization modifications were separable events. We hypothesized that this latter activity might contribute to maintenance of the methylation imprint in early embryos. Here, we demonstrate that methylation of the paternally inherited transgenic H19 ICR commences soon after fertilization in a maternal DNMT3A- and DNMT3L-dependent manner. When its germline methylation was partially obstructed by insertion of insulator sequences, the endogenous paternal H19 ICR also exhibited postfertilization methylation. Finally, we refined the responsible sequences for this activity in transgenic mice and found that deletion of the 5' segment of the endogenous paternal H19 ICR decreased its methylation after fertilization and attenuated Igf2 gene expression. These results demonstrate that this segment of the H19 ICR is essential for its de novo postfertilization DNA methylation, and that this activity contributes to the maintenance of imprinted methylation at the endogenous H19 ICR during early embryogenesis.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Blotting, Southern (日) (読) [継承]
4. (英) DNA (Cytosine-5-)-Methyltransferase (日) (読) [継承]
5. (英) DNA Methylation (日) (読) [継承]
6. (英) DNA Primers (日) (読) [継承]
7. (英) Embryonic Development (日) (読) [継承]
8. (英) Female (日) (読) [継承]
9. (英) Gene Expression Regulation, Developmental (日) (読) [継承]
10. (英) Genomic Imprinting (日) (読) [継承]
11. (英) Insulin-Like Growth Factor II (日) (読) [継承]
12. (英) Male (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Mice, Transgenic (日) (読) [継承]
15. (英) Molecular Sequence Data (日) (読) [継承]
16. (英) RNA, Long Noncoding (日) (読) [継承]
17. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
18. (英) Sequence Analysis, DNA (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Development ([The Company of Biologists Limited])
(pISSN: 0950-1991, eISSN: 1477-9129)

ISSN (任意): 1477-9129
ISSN: 0950-1991 (pISSN: 0950-1991, eISSN: 1477-9129)
Title: Development (Cambridge, England)
Title(ISO): Development
Publisher: Company of Biologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 142 [継承]
(必須): 22 [継承]
(必須): 3833 3844 [継承]
都市 (任意):
年月日 (必須): 西暦 2015年 9月 28日 (平成 27年 9月 28日) [継承]
URL (任意):
DOI (任意): 10.1242/dev.126003    (→Scopusで検索) [継承]
PMID (任意): 26417043    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意): 000366361300005 [継承]
Scopus (任意): 2-s2.0-84947483852 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1242/dev.126003  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) KeywordList.Keyword: DNA methylation  (日)    [継承]
5.(英) KeywordList.Keyword: Early embryogenesis  (日)    [継承]
6.(英) KeywordList.Keyword: Genomic imprinting  (日)    [継承]
7.(英) KeywordList.Keyword: Igf2/H19 locus  (日)    [継承]

標準的な表示

和文冊子 ● Matsuzaki HItomi, Eiichi Okamura, Takahashi Takuya, Ushiki Aki, Nakamura Toshinobu, Nakano Toru, Hata Kenichiro, Fukamizu Akiyoshi and Tanimoto Keiji : De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis., Development, Vol.142, No.22, 3833-3844, 2015.
欧文冊子 ● Matsuzaki HItomi, Eiichi Okamura, Takahashi Takuya, Ushiki Aki, Nakamura Toshinobu, Nakano Toru, Hata Kenichiro, Fukamizu Akiyoshi and Tanimoto Keiji : De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis., Development, Vol.142, No.22, 3833-3844, 2015.

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