『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=306315EID:306315, Map:0, LastModified:2018年3月13日(火) 15:34:07, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[井本 逸勢], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
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著者 (必須): 1.村田 知慧
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2. (英) Sawaya Hirohito (日) (読)
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3. (英) Nakata Katsushi (日) (読)
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4. (英) Yamada Fumio (日) (読)
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5.井本 逸勢
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6. (英) Kuroiwa Asato (日) (読)
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題名 (必須): (英) The cryptic Y-autosome translocation in the small Indian mongoose, Herpestes auropunctatus, revealed by molecular cytogenetic approaches.  (日)    [継承]
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要約 (任意): (英) In initial studies of the eutherian small Indian mongoose (Herpestes auropunctatus), the Y chromosome could not be identified in somatic cells. The male chromosome number is uniquely odd, 2n = 35, whereas that of females is 2n = 36. Previous reports indicated that this unique karyotype resulted from a translocation of the ancestral Y chromosome to an autosome. However, it has been difficult to identify the chromosomes that harbor the translocated Y chromosomal segment because it is an extremely small euchromatic region. Using a Southern blot analysis, we detected four conserved Y-linked genes, SRY, EIF2S3Y, KDM5D, and ZFY, in the male genome. We cloned homologues of these genes and determined their sequences, which showed high homology to genes in two carnivore species, cat and dog. To unambiguously identify the Y-bearing autosome, we performed immunostaining of pachytene spermatocytes using antibodies against SYCP3, γH2AX, and the centromere. We observed trivalent chromosomes, and the associations between the distal ends of the chromosomes were consistent with those of Y and X1 chromosomes. The centromere of the Y chromosome was located on the ancestral Y chromosomal segment. We mapped the complementary DNA (cDNA) clones of these genes to the male chromosomes using fluorescence in situ hybridization (FISH), and the linear localization of all genes was confirmed by two-colored FISH. These Y-linked genes were localized to the proximal region of the long arm of a single telomeric chromosome, and we successfully identified the chromosome harboring the ancestral Y chromosomal segment.  (日)    [継承]
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誌名 (必須): Chromosoma ([Springer-Verlag])
(pISSN: 0009-5915, eISSN: 1432-0886)

ISSN (任意): 1432-0886
ISSN: 0009-5915 (pISSN: 0009-5915, eISSN: 1432-0886)
Title: Chromosoma
Title(ISO): Chromosoma
Supplier: Springer Online Journal Archive
Publisher: Springer Berlin Heidelberg
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年月日 (必須): 西暦 2016年 1月 7日 (平成 28年 1月 7日) [継承]
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DOI (任意): 10.1007/s00412-015-0572-3    (→Scopusで検索) [継承]
PMID (任意): 26743516    (→Scopusで検索) [継承]
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WOS (任意): 000383464800017 [継承]
Scopus (任意): 2-s2.0-84953408393 [継承]
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備考 (任意): 1.(英) PublicationType: JOURNAL ARTICLE  (日)    [継承]

標準的な表示

和文冊子 ● Chie Murata, Hirohito Sawaya, Katsushi Nakata, Fumio Yamada, Issei Imoto and Asato Kuroiwa : The cryptic Y-autosome translocation in the small Indian mongoose, Herpestes auropunctatus, revealed by molecular cytogenetic approaches., Chromosoma, (巻), (号), (頁), 2016.
欧文冊子 ● Chie Murata, Hirohito Sawaya, Katsushi Nakata, Fumio Yamada, Issei Imoto and Asato Kuroiwa : The cryptic Y-autosome translocation in the small Indian mongoose, Herpestes auropunctatus, revealed by molecular cytogenetic approaches., Chromosoma, (巻), (号), (頁), 2016.

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