『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=305549)

EID=305549EID:305549, Map:0, LastModified:2016年2月1日(月) 10:48:57, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[宮本 理人], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.宮本 理人
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Egawa Tatsuro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Oshima Rieko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Kurogi Eriko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Tomida Yosuke (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Tsuchiya Koichiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Hayashi Tatsuya (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) AICAR stimulation metabolome-widely mimics electrical contraction in isolated rat epitrochlearis muscle.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Physical exercise has potent therapeutic and preventive effects against metabolic disorders. A number of studies have suggested that 5'-AMP-activated protein kinase (AMPK) plays a pivotal role in regulating carbohydrate and lipid metabolism in contracting skeletal muscles, while several genetically manipulated animal models revealed the significance of AMPK-independent pathways. To elucidate significance of AMPK and AMPK-independent signals in contracting skeletal muscles, we conducted a metabolomic analysis that compared the metabolic effects of 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside (AICAR) stimulation with the electrical contraction ex vivo in isolated rat epitrochlearis muscles, in which both α1- and α2-isoforms of AMPK and glucose uptake were equally activated. The metabolomic analysis using capillary electrophoresis time-of-flight mass spectrometry detected 184 peaks and successfully annotated 132 small molecules. AICAR stimulation exhibited high similarity to the electrical contraction in overall metabolites. Principal component analysis (PCA) demonstrated that the major principal component characterized common effects whereas the minor principal component distinguished the difference. PCA and a factor analysis suggested a substantial change in redox status as a result of AMPK activation. We also found a decrease in reduced glutathione levels in both AICAR-stimulated and contracting muscles. The muscle contraction-evoked influences related to the metabolism of amino acids, in particular, aspartate, alanine, or lysine, are supposed to be independent of AMPK activation. Our results substantiate the significance of AMPK activation in contracting skeletal muscles and provide novel evidence that AICAR stimulation closely mimics the metabolomic changes in the contracting skeletal muscles.  (日)    [継承]
キーワード (推奨): 1. (英) AMP-Activated Protein Kinases (日) (読) [継承]
2. (英) Aminoimidazole Carboxamide (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Cell Line (日) (読) [継承]
5. (英) Electric Stimulation (日) (読) [継承]
6. (英) Factor Analysis, Statistical (日) (読) [継承]
7.グルコース (glucose) [継承]
8. (英) Glutathione (日) (読) [継承]
9. (英) Hypoglycemic Agents (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Metabolome (日) (読) [継承]
12. (英) Muscle Contraction (日) (読) [継承]
13. (英) Muscle, Skeletal (日) (読) [継承]
14. (英) Principal Component Analysis (日) (読) [継承]
15. (英) Random Allocation (日) (読) [継承]
16. (英) Rats (日) (読) [継承]
17. (英) Rats, Sprague-Dawley (日) (読) [継承]
18. (英) Ribonucleotides (日) (読) [継承]
19. (英) Transcriptome (日) (読) [継承]
発行所 (推奨):
誌名 (必須): American Journal of Physiology, Cell Physiology ([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)

ISSN (任意): 1522-1563
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 305 [継承]
(必須): 12 [継承]
(必須): 1214 1222 [継承]
都市 (任意):
年月日 (必須): 西暦 2014年 1月 14日 (平成 26年 1月 14日) [継承]
URL (任意):
DOI (任意): 10.1152/ajpcell.00162.2013    (→Scopusで検索) [継承]
PMID (任意): 24088893    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000328760800005 [継承]
Scopus (任意): 2-s2.0-84890390626 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1152/ajpcell.00162.2013  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) KeywordList.Keyword: 5'-AMP-activated protein kinase  (日)    [継承]
5.(英) KeywordList.Keyword: AICAR  (日)    [継承]
6.(英) KeywordList.Keyword: isolated skeletal muscle  (日)    [継承]
7.(英) KeywordList.Keyword: metabolome  (日)    [継承]
8.(英) KeywordList.Keyword: muscle contraction  (日)    [継承]

標準的な表示

和文冊子 ● Licht Miyamoto, Tatsuro Egawa, Rieko Oshima, Eriko Kurogi, Yosuke Tomida, Koichiro Tsuchiya and Tatsuya Hayashi : AICAR stimulation metabolome-widely mimics electrical contraction in isolated rat epitrochlearis muscle., American Journal of Physiology, Cell Physiology, Vol.305, No.12, 1214-1222, 2014.
欧文冊子 ● Licht Miyamoto, Tatsuro Egawa, Rieko Oshima, Eriko Kurogi, Yosuke Tomida, Koichiro Tsuchiya and Tatsuya Hayashi : AICAR stimulation metabolome-widely mimics electrical contraction in isolated rat epitrochlearis muscle., American Journal of Physiology, Cell Physiology, Vol.305, No.12, 1214-1222, 2014.

関連情報

Number of session users = 4, LA = 0.91, Max(EID) = 383948, Max(EOID) = 1026951.