| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | 山本 武範
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| 2. | 松尾 泰佑
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| 3. | (英) Yamamoto Atsushi (日) (読)
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| 4. | (英) Yamagoshi Ryohei (日) (読)
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| 5. | 大倉 一人 ([名古屋大学])
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| 6. | 片岡 正俊
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| 7. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane. (日)
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| ○要約 (任意): | □ | (英) Immunodetection using antibodies, e.g., Western blotting, is generally utilized to measure the amount of a certain protein in a protein mixture. For valid interpretation of results observed by immunodetection, strict attention must be paid to the factors affecting the immunoreactivities of the antibodies. We here describe the step-by-step procedures to demonstrate that substitution of certain amino acids in a peptide can cause remarkable differences in its immunoreactivity with antibodies against epitope tags in the immobilized peptide. Refolding of the peptide on the membrane in a way that masks the epitope to different degrees was the possible reason for their distinct immunoreactivities with the antibodies. The results in this chapter suggest that we need to interpret carefully the experimental results involving immunodetection. (日)
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| ○誌名 (必須): | □ | Methods in Molecular Biology ([Springer])
(pISSN: 1064-3745, eISSN: 1940-6029)
| ○ISSN (任意): | □ | 1940-6029
ISSN: 1064-3745
(pISSN: 1064-3745, eISSN: 1940-6029) Title: Methods in molecular biology (Clifton, N.J.)Title(ISO): Methods Mol BiolPublisher: Humana Press Inc. (NLM Catalog)
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| ○巻 (必須): | □ | 1348
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 303 310
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| ○年月日 (必須): | □ | 西暦 2015年 0月 初日 (平成 27年 0月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/978-1-4939-2999-3_26 (→Scopusで検索)
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| ○PMID (任意): | □ | 26424282 (→Scopusで検索)
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| ○Scopus (任意): | □ | 2-s2.0-84942875435
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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