○種別 (必須): | □ | 学術論文 (審査論文)
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○言語 (必須): | □ | 英語
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○招待 (推奨): |
○審査 (推奨): |
○カテゴリ (推奨): |
○共著種別 (推奨): |
○学究種別 (推奨): |
○組織 (推奨): |
○著者 (必須): | 1. | (英) Ikawa H (日) (読)
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| 2. | 山本 圭 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生命科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
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| 3. | (英) Takahashi Y (日) (読)
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| 4. | (英) Ueda N (日) (読)
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| 5. | (英) Hayashi Y (日) (読)
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| 6. | (英) Yamamoto S (日) (読)
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| 7. | (英) Ishimura K (日) (読)
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| 8. | (英) Irahara M (日) (読)
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| 9. | (英) Aono T (日) (読)
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○題名 (必須): | □ | (英) Arachidonate 12-lipoxygenase in porcine anterior pituitary cells; its localization and possible function in gonadotrophs. (日)
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○副題 (任意): |
○要約 (任意): | □ | (英) Arachidonate 12-lipoxygenase, which oxygenates positions 12 and 13 of arachidonic and linoleic acids, is present in porcine anterior pituitary cells. Colocalization of the 12-lipoxygenase with various pituitary hormones was examined by immunohistochemical double-staining using antibodies against 12-lipoxygenase and various anterior pituitary hormones. Under light microscopy, approximately 7% of the cells producing luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were positive for 12-lipoxygenase, whereas the enzyme was detected in less than 2% of the cells producing thyrotrophin, prolactin, growth hormone (GH), and adrenocorticotrophin. In an attempt to examine the participation of 12-lipoxygenase metabolites in pituitary hormone release, we incubated the primary culture of porcine anterior pituitary cells with 12-hydroperoxy-arachidonic acid or 13-hydroperoxy-linoleic acid. Significant stimulation of LH and FSH release by these hydroperoxides was observed at 10 microM in a time-dependent manner. At doses around 10 microM these compounds produced responses of similar magnitude to 1 nM gonadotrophin-releasing hormone (GnRH), but higher concentrations (30 microM) of the compounds were required for GH release. In contrast, 12-hydroxy-arachidonic and 13-hydroxy-linoleic acids were almost ineffective. Furthermore, the gonadotrophin release by 1 nM GnRH was inhibited by nordihydroguaiaretic acid (a lipoxygenase inhibitor) with an IC50 of about 5 microM. Thus, the hydroperoxy (but not hydroxy) products of 12-lipoxygenase may be involved in the release of pituitary hormones especially LH and FSH. (日)
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○キーワード (推奨): | 1. | (英) 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid (日) (読)
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| 2. | (英) Animals (日) (読)
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| 3. | (英) Antithrombins (日) (読)
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| 4. | (英) Arachidonate 12-Lipoxygenase (日) (読)
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| 5. | (英) Cells, Cultured (日) (読)
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| 6. | (英) Chromatography, High Pressure Liquid (日) (読)
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| 7. | (英) Fatty Acids, Unsaturated (日) (読)
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| 8. | (英) Follicle Stimulating Hormone (日) (読)
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| 9. | (英) Gonadotropin-Releasing Hormone (日) (読)
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| 10. | (英) Growth Hormone (日) (読)
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| 11. | (英) Hydroxyeicosatetraenoic Acids (日) (読)
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| 12. | 免疫組織化学 (immunohistochemistry)
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| 13. | (英) Leukotrienes (日) (読)
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| 14. | (英) Linoleic Acids (日) (読)
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| 15. | (英) Lipid Peroxides (日) (読)
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| 16. | (英) Luteinizing Hormone (日) (読)
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| 17. | (英) Pituitary Gland, Anterior (日) (読)
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| 18. | (英) Swine (日) (読)
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| 19. | (英) Time Factors (日) (読)
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○発行所 (推奨): |
○誌名 (必須): | □ | The Journal of Endocrinology (Society for Endocrinology)
(pISSN: 0022-0795, eISSN: 1479-6805)
○ISSN (任意): | □ | 0022-0795
ISSN: 0022-0795
(pISSN: 0022-0795, eISSN: 1479-6805) Title: The Journal of endocrinologyTitle(ISO): J EndocrinolPublisher: Society for Endocrinology (NLM Catalog)
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(CrossRef)
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○巻 (必須): | □ | 148
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○号 (必須): | □ |
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○頁 (必須): | □ | 33 41
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○都市 (任意): |
○年月日 (必須): | □ | 西暦 1996年 1月 初日 (平成 8年 1月 初日)
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○URL (任意): |
○DOI (任意): | □ | 10.1677/joe.0.1480033 (→Scopusで検索)
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○PMID (任意): | □ | 8568469 (→Scopusで検索)
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○CRID (任意): |
○Scopus (任意): | □ | 2-s2.0-0030033696
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○備考 (任意): | 1. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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