『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=300735)

EID=300735EID:300735, Map:0, LastModified:2016年4月6日(水) 13:27:38, Operator:[三好 小文], Avail:TRUE, Censor:0, Owner:[川上 竜巳], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Ohmori Taketo (日) 大森 勇門 (読) おおもり たけと
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3.櫻庭 春彦
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4.大島 敏久 ([九州大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids  (日)    [継承]
副題 (任意):
要約 (任意): (英) To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Amino Acids ([Springer])
(pISSN: 0939-4451, eISSN: 1438-2199)

ISSN (任意): 1438-2199
ISSN: 0939-4451 (pISSN: 0939-4451, eISSN: 1438-2199)
Title: Amino acids
Title(ISO): Amino Acids
Supplier: Springer Online Journal Archive
Publisher: Springer Wien
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 47 [継承]
(必須): 8 [継承]
(必須): 1579 1587 [継承]
都市 (任意):
年月日 (必須): 西暦 2015年 8月 初日 (平成 27年 8月 初日) [継承]
URL (任意):
DOI (任意): 10.1007/s00726-015-2001-6    (→Scopusで検索) [継承]
PMID (任意): 25963389    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000358175100009 [継承]
Scopus (任意): 2-s2.0-84937518494 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1007/s00726-015-2001-6  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Ryushi Kawakami, Taketo Ohmori, Haruhiko Sakuraba and Toshihisa Ohshima : Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids, Amino Acids, Vol.47, No.8, 1579-1587, 2015.
欧文冊子 ● Ryushi Kawakami, Taketo Ohmori, Haruhiko Sakuraba and Toshihisa Ohshima : Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids, Amino Acids, Vol.47, No.8, 1579-1587, 2015.

関連情報

Number of session users = 1, LA = 0.69, Max(EID) = 377831, Max(EOID) = 1011135.