『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=300231)

EID=300231EID:300231, Map:0, LastModified:2015年9月4日(金) 15:12:29, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[岡村 永一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.岡村 永一
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Matsuzaki Hitomi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Fukamizu Akiyoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Tanimoto Keiji (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) The chicken HS4 insulator element does not protect the H19 ICR from differential DNA methylation in yeast artificial chromosome transgenic mouse.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Mono-allelic expression at the mouse IGF2/H19 locus is controlled by differential allelic DNA methylation of the imprinting control region (ICR). Because a randomly integrated H19 ICR fragment, when incorporated into the genome of transgenic mice (TgM), was allele-specifically methylated in somatic, but not in germ cells, it was suggested that allele-discriminating epigenetic signature, set within or somewhere outside of the Tg H19 ICR fragment in germ cells, was later translated into a differential DNA methylation pattern. To test if the chicken β-globin HS4 (cHS4) chromatin insulator might interfere with methylation imprinting establishment at the H19 ICR, we inserted the H19 ICR fragment, flanked by a set of floxed cHS4 core sequences, into a human β-globin locus YAC and generated TgM (insulated ICR' TgM). As controls, the cHS4 sequences were removed from one side (5'HS4-deleted ICR') or both sides (pseudo-WT ICR') of the insulated ICR' by in vivo cre-loxP recombination. The data show that while maternally inherited transgenic H19 ICR was not methylated in insulated ICR' TgM, it was significantly methylated upon paternal transmission, though the level was lower than in the pseudo-WT ICR' control. Because this reduced level of methylation was also observed in the 5'HS4-deleted ICR' TgM, we speculate that the phenotype is due to VEZF1-dependent demethylation activity, rather than the insulator function, borne in cHS4. Collectively, although we cannot rule out the possibility that cHS4 is incapable of blocking an allele-discriminating signal from outside of the transgene, the epigenetic signature appears to be marked intrinsically within the H19 ICR.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Chickens (日) (読) [継承]
3. (英) Chromosomes, Artificial (日) (読) [継承]
4. (英) DNA Methylation (日) (読) [継承]
5. (英) Genomic Imprinting (日) (読) [継承]
6. (英) Humans (日) (読) [継承]
7. (英) Insulator Elements (日) (読) [継承]
8. (英) Insulin-Like Growth Factor II (日) (読) [継承]
9. (英) Mice (日) (読) [継承]
10. (英) Mice, Transgenic (日) (読) [継承]
11. (英) Saccharomyces cerevisiae (日) (読) [継承]
発行所 (推奨):
誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 8 [継承]
(必須): 9 [継承]
(必須): e73925 e73925 [継承]
都市 (任意):
年月日 (必須): 西暦 2013年 9月 4日 (平成 25年 9月 4日) [継承]
URL (任意):
DOI (任意): 10.1371/journal.pone.0073925    (→Scopusで検索) [継承]
PMID (任意): 24023912    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.pone.0073925  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC3762768  (日)    [継承]

標準的な表示

和文冊子 ● Eiichi Okamura, Hitomi Matsuzaki, Akiyoshi Fukamizu and Keiji Tanimoto : The chicken HS4 insulator element does not protect the H19 ICR from differential DNA methylation in yeast artificial chromosome transgenic mouse., PLoS ONE, Vol.8, No.9, e73925, 2013.
欧文冊子 ● Eiichi Okamura, Hitomi Matsuzaki, Akiyoshi Fukamizu and Keiji Tanimoto : The chicken HS4 insulator element does not protect the H19 ICR from differential DNA methylation in yeast artificial chromosome transgenic mouse., PLoS ONE, Vol.8, No.9, e73925, 2013.

関連情報

Number of session users = 1, LA = 0.29, Max(EID) = 405196, Max(EOID) = 1098796.