『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=300229EID:300229, Map:0, LastModified:2015年9月10日(木) 17:06:48, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[岡村 永一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1.岡村 永一
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2. (英) Matsuzaki Hitomi (日) (読)
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3. (英) Sakaguchi Ryuuta (日) (読)
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4. (英) Takahashi Takuya (日) (読)
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5. (英) Fukamizu Akiyoshi (日) (読)
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6. (英) Tanimoto Keiji (日) (読)
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題名 (必須): (英) The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice.  (日)    [継承]
副題 (任意):
要約 (任意): (英) In the mouse Igf2/H19 imprinted locus, differential methylation of the imprinting control region (H19 ICR) is established during spermatogenesis and is maintained in offspring throughout development. Previously, however, we observed that the paternal H19 ICR, when analyzed in yeast artificial chromosome transgenic mice (YAC-TgM), was preferentially methylated only after fertilization. To identify the DNA sequences that confer methylation imprinting, we divided the H19 ICR into two fragments (1.7 and 1.2 kb), ligated them to both ends of a λ DNA fragment into which CTCF binding sites had been inserted, and analyzed this in YAC-TgM. The maternally inherited λ sequence, normally methylated after implantation in the absence of H19 ICR sequences, became hypomethylated, demonstrating protective activity against methylation within the ICR. Meanwhile, the paternally inherited λ sequence was hypermethylated before implantation only when a 1.7-kb fragment was ligated. Consistently, when two subfragments of the H19 ICR were individually investigated for their activities in YAC-TgM, only the 1.7-kb fragment was capable of introducing paternal allele-specific DNA methylation. These results show that postfertilization methylation imprinting is conferred by a paternal allele-specific methylation activity present in a 1.7-kb DNA fragment of the H19 ICR, while maternal allele-specific activities protect the allele from de novo DNA methylation.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Blastocyst (日) (読) [継承]
4. (英) Chromosomes, Artificial, Yeast (日) (読) [継承]
5. (英) DNA Methylation (日) (読) [継承]
6. (英) Female (日) (読) [継承]
7. (英) Fertilization (日) (読) [継承]
8. (英) Genomic Imprinting (日) (読) [継承]
9. (英) Male (日) (読) [継承]
10. (英) Mice (日) (読) [継承]
11. (英) Mice, Transgenic (日) (読) [継承]
12. (英) Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular and Cellular Biology ([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)

ISSN (任意): 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: Taylor & Francis
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 858 871 [継承]
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年月日 (必須): 西暦 2012年 12月 10日 (平成 24年 12月 10日) [継承]
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DOI (任意): 10.1128/MCB.01003-12    (→Scopusで検索) [継承]
PMID (任意): 23230275    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1128/MCB.01003-12  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC3571351  (日)    [継承]

標準的な表示

和文冊子 ● Eiichi Okamura, Hitomi Matsuzaki, Ryuuta Sakaguchi, Takuya Takahashi, Akiyoshi Fukamizu and Keiji Tanimoto : The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice., Molecular and Cellular Biology, Vol.33, No.4, 858-871, 2012.
欧文冊子 ● Eiichi Okamura, Hitomi Matsuzaki, Ryuuta Sakaguchi, Takuya Takahashi, Akiyoshi Fukamizu and Keiji Tanimoto : The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice., Molecular and Cellular Biology, Vol.33, No.4, 858-871, 2012.

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