『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=300227)

EID=300227EID:300227, Map:0, LastModified:2015年9月10日(木) 17:21:59, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[岡村 永一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Shimotsuma Motoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.岡村 永一
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Matsuzaki Hitomi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Fukamizu Akiyoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Tanimoto Keiji (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) DNase I hypersensitivity and epsilon-globin transcriptional enhancement are separable in locus control region (LCR) HS1 mutant human beta-globin YAC transgenic mice.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Expression of the five beta-like globin genes (epsilon, Ggamma, Agamma, delta, beta) in the human beta-globin locus depends on enhancement by the locus control region, which consists of five DNase I hypersensitive sites (5'HS1 through 5'HS5). We report here a novel enhancer activity in 5'HS1 that appears to be potent in transfected K562 cells. Deletion analyses identified a core activating element that bound to GATA-1, and a two-nucleotide mutation that disrupted GATA-1 binding in vitro abrogated 5'HS1 enhancer activity in transfection experiments. To determine the in vivo role of this GATA site, we generated multiple lines of human beta-globin YAC transgenic mice bearing the same two-nucleotide mutation. In the mutant mice, epsilon-, but not gamma-globin, gene expression in primitive erythroid cells was severely attenuated, while adult beta-globin gene expression in definitive erythroid cells was unaffected. Interestingly, DNaseI hypersensitivity near the 5'HS1 mutant sequence was eliminated in definitive erythroid cells, whereas it was only mildly affected in primitive erythroid cells. We therefore conclude that, although the GATA site in 5'HS1 is critical for efficient epsilon-globin gene expression, hypersensitive site formation per se is independent of 5'HS1 function, if any, in definitive erythroid cells.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Base Sequence (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Chromatin Immunoprecipitation (日) (読) [継承]
5. (英) Chromosomes, Artificial, Yeast (日) (読) [継承]
6. (英) Deoxyribonuclease I (日) (読) [継承]
7. (英) Electrophoretic Mobility Shift Assay (日) (読) [継承]
8. (英) Enhancer Elements, Genetic (日) (読) [継承]
9. (英) Erythroid Cells (日) (読) [継承]
10. (英) GATA1 Transcription Factor (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Locus Control Region (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Mice, Transgenic (日) (読) [継承]
15. (英) Molecular Sequence Data (日) (読) [継承]
16. (英) Mutation (日) (読) [継承]
17. (英) Promoter Regions, Genetic (日) (読) [継承]
18. (英) RNA, Messenger (日) (読) [継承]
19. (英) Regulatory Sequences, Nucleic Acid (日) (読) [継承]
20. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
21. (英) Sequence Deletion (日) (読) [継承]
22. (英) Sequence Homology, Nucleic Acid (日) (読) [継承]
23. (英) Transcription, Genetic (日) (読) [継承]
24. (英) beta-Globins (日) (読) [継承]
25. (英) epsilon-Globins (日) (読) [継承]
26. (英) gamma-Globins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 1083-351X
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 285 [継承]
(必須): 19 [継承]
(必須): 14495 14503 [継承]
都市 (任意):
年月日 (必須): 西暦 2010年 3月 15日 (平成 22年 3月 15日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.M110.116525    (→Scopusで検索) [継承]
PMID (任意): 20231293    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1074/jbc.M110.116525  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2863201  (日)    [継承]

標準的な表示

和文冊子 ● Motoshi Shimotsuma, Eiichi Okamura, Hitomi Matsuzaki, Akiyoshi Fukamizu and Keiji Tanimoto : DNase I hypersensitivity and epsilon-globin transcriptional enhancement are separable in locus control region (LCR) HS1 mutant human beta-globin YAC transgenic mice., The Journal of Biological Chemistry, Vol.285, No.19, 14495-14503, 2010.
欧文冊子 ● Motoshi Shimotsuma, Eiichi Okamura, Hitomi Matsuzaki, Akiyoshi Fukamizu and Keiji Tanimoto : DNase I hypersensitivity and epsilon-globin transcriptional enhancement are separable in locus control region (LCR) HS1 mutant human beta-globin YAC transgenic mice., The Journal of Biological Chemistry, Vol.285, No.19, 14495-14503, 2010.

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