『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=300224)

EID=300224EID:300224, Map:0, LastModified:2015年9月12日(土) 19:35:36, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[岡村 永一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Matsuzaki Hitomi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.岡村 永一
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Shimotsuma Motoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Fukamizu Akiyoshi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Tanimoto Keiji (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The imprinted expression of the mouse Igf2/H19 locus is governed by the differential methylation of the imprinting control region (ICR), which is established initially in germ cells and subsequently maintained in somatic cells, depending on its parental origin. By grafting a 2.9-kbp H19 ICR fragment into a human beta-globin yeast artificial chromosome in transgenic mice, we previously showed that the ICR could recapitulate imprinted methylation and expression at a heterologous locus, suggesting that the H19 ICR in the beta-globin locus contained sufficient information to maintain the methylation mark (K. Tanimoto, M. Shimotsuma, H. Matsuzaki, A. Omori, J. Bungert, J. D. Engel, and A. Fukamizu, Proc. Natl. Acad. Sci. USA 102:10250-10255, 2005). Curiously, however, the transgenic H19 ICR was not methylated in sperm, which was distinct from that seen in the endogenous locus. Here, we reevaluated the ability of the H19 ICR to mark the parental origin using more rigid criteria. In the testis, the methylation levels of the solitary 2.9-kbp transgenic ICR fragment varied significantly between six transgenic mouse lines. However, in somatic cells, the paternally inherited ICR fragment exhibited consistently higher methylation levels at five out of six randomly integrated sites in the mouse genome. These results clearly demonstrated that the H19 ICR could acquire parent-of-origin-dependent methylation after fertilization independently of the chromosomal integration site or the prerequisite methylation acquisition in male germ cells.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Cells, Cultured (日) (読) [継承]
3. (英) DNA Methylation (日) (読) [継承]
4. (英) Female (日) (読) [継承]
5. (英) Genomic Imprinting (日) (読) [継承]
6. (英) Germ Cells (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8. (英) Insulin-Like Growth Factor II (日) (読) [継承]
9. (英) Locus Control Region (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Mice (日) (読) [継承]
12. (英) Mice, Transgenic (日) (読) [継承]
13. (英) Pedigree (日) (読) [継承]
14. (英) RNA, Long Noncoding (日) (読) [継承]
15. (英) RNA, Untranslated (日) (読) [継承]
16. (英) Testis (日) (読) [継承]
17. (英) Transgenes (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular and Cellular Biology ([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)

ISSN (任意): 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: Taylor & Francis
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 29 [継承]
(必須): 17 [継承]
(必須): 4595 4603 [継承]
都市 (任意):
年月日 (必須): 西暦 2009年 6月 22日 (平成 21年 6月 22日) [継承]
URL (任意):
DOI (任意): 10.1128/MCB.00275-09    (→Scopusで検索) [継承]
PMID (任意): 19546235    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1128/MCB.00275-09  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC2725707  (日)    [継承]

標準的な表示

和文冊子 ● Hitomi Matsuzaki, Eiichi Okamura, Motoshi Shimotsuma, Akiyoshi Fukamizu and Keiji Tanimoto : A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells., Molecular and Cellular Biology, Vol.29, No.17, 4595-4603, 2009.
欧文冊子 ● Hitomi Matsuzaki, Eiichi Okamura, Motoshi Shimotsuma, Akiyoshi Fukamizu and Keiji Tanimoto : A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells., Molecular and Cellular Biology, Vol.29, No.17, 4595-4603, 2009.

関連情報

Number of session users = 0, LA = 0.64, Max(EID) = 405196, Max(EOID) = 1098796.