『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=298307EID:298307, Map:0, LastModified:2015年9月9日(水) 21:37:46, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[宇都 義浩], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Ueno Megumi (日) (読)
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2. (英) Nyui Minako (日) (読)
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3. (英) Nakanishi Ikuo (日) (読)
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4. (英) Anzai Kazunori (日) (読)
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5. (英) Ozawa Toshihiko (日) (読)
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6. (英) Matsumoto Ken-ichiro (日) (読)
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7.宇都 義浩 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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題名 (必須): (英) Scavenging of reactive oxygen species induced by hyperthermia in biological fluid  (日)    [継承]
副題 (任意):
要約 (任意): (英) The scavenging activity of rat plasma against hyperthermia-induced reactive oxygen species was tested. The glutathione-dependent reduction of a nitroxyl radical, 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, which was restricted by adding superoxide dismutase or by deoxygenating the reaction mixture, was applied to an index of superoxide (O2 (·-)) generation. A reaction mixture containing 0.1 mM 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl and 1 mM glutathione was prepared using 100 mM phosphate buffer containing 0.05 mM diethylenetriaminepentaacetic acid. The reaction mixture was kept in a screw-top vial and incubated in a water bath at 37 or 44°C. The time course of the electron paramagnetic resonance signal of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). When the same experiment was performed using rat plasma instead of 100 mM PB, the glutathione-dependent reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, i.e., generation of O2 (·-), was not obtained. Only the first-order decay reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, which indicates direct reduction of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl, was obtained in rat plasma. Adding 0.5% albumin to the phosphate buffer reaction mixture could almost completely inhibit O2 (·-) generation at 37°C. However, addition of 0.5% albumin could not inhibit O2 (·-) generation at 44°C, i.e., hyperthermic temperature. Ascorbic acid also showed inhibition of O2 (·-) generation by 0.01 mM at 37°C, but 0.02 mM or more could inhibit O2 (·-) generation at 44°C. A higher concentration of ascorbic acid showed first-order reduction, i.e., direct one-electron reduction, of 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl. Hyperthermia-induced O2 (·-) generation in rat plasma can be mostly inhibited by albumin and ascorbic acid in the plasma.  (日)    [継承]
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誌名 (必須): Journal of Clinical Biochemistry and Nutrition (日本酸化ストレス学会)
(pISSN: 0912-0009, eISSN: 1880-5086)

ISSN (任意): 0912-0009
ISSN: 0912-0009 (pISSN: 0912-0009, eISSN: 1880-5086)
Title: Journal of clinical biochemistry and nutrition
Title(ISO): J Clin Biochem Nutr
Supplier: 日本酸化ストレス学会 JCBN事務局
Publisher: Institute of Applied Biochemistry
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2014年 3月 1日 (平成 26年 3月 1日) [継承]
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DOI (任意): 10.3164/jcbn.13-61    (→Scopusで検索) [継承]
PMID (任意): 24688214    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.3164/jcbn.13-61  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) OtherID: PMC3947970  (日)    [継承]
4.(英) KeywordList.Keyword: electron paramagnetic resonance  (日)    [継承]
5.(英) KeywordList.Keyword: hyperthermia  (日)    [継承]
6.(英) KeywordList.Keyword: nitroxyl redox probe  (日)    [継承]
7.(英) KeywordList.Keyword: reactive oxygen species  (日)    [継承]
8.(英) KeywordList.Keyword: superoxide  (日)    [継承]

標準的な表示

和文冊子 ● Megumi Ueno, Minako Nyui, Ikuo Nakanishi, Kazunori Anzai, Toshihiko Ozawa, Ken-ichiro Matsumoto and Yoshihiro Uto : Scavenging of reactive oxygen species induced by hyperthermia in biological fluid, Journal of Clinical Biochemistry and Nutrition, Vol.54, No.2, 75-80, 2014.
欧文冊子 ● Megumi Ueno, Minako Nyui, Ikuo Nakanishi, Kazunori Anzai, Toshihiko Ozawa, Ken-ichiro Matsumoto and Yoshihiro Uto : Scavenging of reactive oxygen species induced by hyperthermia in biological fluid, Journal of Clinical Biochemistry and Nutrition, Vol.54, No.2, 75-80, 2014.

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