| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| 2. | 板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 3. | 稲垣 裕司 ([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 4. | 木戸 玲子
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| 5. | 片岡 正俊
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| 6. | 永田 俊彦
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| 7. | 木戸 淳一
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| ○題名 (必須): | □ | (英) Effect of Hangeshashinto on calprotectin expression in human oral epithelial cells. (日)
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| ○要約 (任意): | □ | (英) (日) Oral epithelial cells produce antimicrobial peptides (AMPs) to prevent microbial infection. Calprotectin (S100A8/S100A9) is one of these AMPs in oral epithelial cells, the expression of which is up-regulated by interleukin-1α (IL-1α). Hangeshashinto (HST) is a traditional Japanese herbal medicine that has anti-inflammatory effects. The purpose of this study was to investigate the effect of HST on the expression of calprotectin through the regulation of IL-1α in oral epithelial cells. Human oral epithelial cells (TR146) were cultured with HST in the presence or absence of anti-IL-1α antibody or IL-1 receptor antagonist, or with six major components of HST (3,4-dihydroxybenzaldehyde, baicalin, ginsenoside Rb1, glycyrrhizin, oleanolic acid and berberine). The expression of S100A8, S100A9, other AMPs and cytokine mRNAs was examined by RT-PCR and quantitative real-time PCR. Calprotectin expression and IL-1α secretion were investigated by ELISA. HST (6 μg/ml) increased the expression of S100A8/S100A9 mRNAs and calprotectin protein, and also up-regulated β-defensin 2 (DEFB4) and S100A7 expression. The expression of IL-1α mRNA and its protein was slightly but significantly increased by HST. A neutralizing antibody against IL-1α and IL-1 receptor antagonist inhibited HST-up-regulated S100A8/S100A9 mRNA expression. Although 3,4-dihydroxybenzaldehyde, baicalin and ginsenoside Rb1 as HST components increased S100A8/S100A9 expression, oleanolic acid and berberine decreased their expression. These results suggest that HST increases the expression of calprotectin, DEFB4 and S100A7 in oral epithelial cells. In response to HST, up-regulation of calprotectin expression may be partially induced via IL-1α.
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Odontology (日本歯科大学歯学会)
(pISSN: 1618-1247, eISSN: 1618-1255)
| ○ISSN (任意): | □ | 1618-1255
ISSN: 1618-1247
(pISSN: 1618-1247, eISSN: 1618-1255) Title: OdontologyTitle(ISO): OdontologyPublisher: Springer (NLM Catalog)
(医中誌Web)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 104
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| ○号 (必須): | □ | 2
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| ○頁 (必須): | □ | 152 162
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| ○年月日 (必須): | □ | 西暦 2016年 5月 初日 (平成 28年 5月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s10266-015-0196-3 (→Scopusで検索)
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| ○PMID (任意): | □ | 25649126 (→Scopusで検索)
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| ○WOS (任意): | □ | 000374310000004
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| ○備考 (任意): | 1. | (英) PublicationType: JOURNAL ARTICLE (日)
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