『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=296654EID:296654, Map:0, LastModified:2016年2月25日(木) 14:38:34, Operator:[岡村 裕彦], Avail:TRUE, Censor:0, Owner:[寺町 順平], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Shinohara Hiroki (日) (読)
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2.寺町 順平
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3.岡村 裕彦
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4.楊 諦
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5.永田 俊彦
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6.羽地 達次
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題名 (必須): (英) Double stranded RNA-dependent protein kinase is necessary for TNF--induced osteoclast formation in vitro and in vivo.  (日)    [継承]
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要約 (任意): (英) Double-stranded RNA-dependent protein kinase (PKR) is involved in cell cycle progression, cell proliferation, cell differentiation, tumorgenesis, and apoptosis. We previously reported that PKR is required for differentiation and calcification in osteoblasts. TNF- plays a key role in osteoclast differentiation. However, it is unknown about the roles of PKR in the TNF--induced osteoclast differentiation. The expression of PKR in osteoclast precursor RAW264.7 cells increased during TNF--induced osteoclastogenesis. The TNF--induced osteoclast differentiation in bone marrow-derived macrophages and RAW264.7 cells was markedly suppressed by the pre-treatment of PKR inhibitor, 2-aminopurine (2AP), as well as gene silencing of PKR. The expression of gene markers in the differentiated osteoclasts including TRAP, Calcitonin receptor, cathepsin K and ATP6V0d2 was also suppressed by the 2AP treatment. Bone resorption activity of TNF--induced osteoclasts was also supressed by 2AP treatment. Inhibition of PKR supressed the TNF--induced activation of NF-B and MAPK in RAW264.7 cells. 2AP inhibited both the nuclear translocation of NF-B and its transcriptional activity in RAW264.7 cells. 2AP inhibited the TNF--induced expression of NFATc1 and c-fos, master transcription factors in osteoclastogenesis. TNF--induced nuclear translocation of NFATc1 in mature osteoclasts was clearly inhibited by the 2AP treatment. The PKR inhibitor C16 decreased the TNF--induced osteoclast formation and bone resorption in mouse calvaria. The present study indicates that PKR is necessary for the TNF--induced osteoclast differentiation in vitro and in vivo. This article is protected by copyright. All rights reserved.  (日)    [継承]
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誌名 (必須): Journal of Cellular Biochemistry ([Wiley-Liss, Inc.])
(pISSN: 0730-2312, eISSN: 1097-4644)

ISSN (任意): 1097-4644
ISSN: 0730-2312 (pISSN: 0730-2312, eISSN: 1097-4644)
Title: Journal of cellular biochemistry
Title(ISO): J Cell Biochem
Publisher: Wiley
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(必須): 116 [継承]
(必須): 9 [継承]
(必須): 1957 1967 [継承]
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年月日 (必須): 西暦 2015年 9月 初日 (平成 27年 9月 初日) [継承]
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DOI (任意): 10.1002/jcb.25151    (→Scopusで検索) [継承]
PMID (任意): 25739386    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: JOURNAL ARTICLE  (日)    [継承]

標準的な表示

和文冊子 ● Hiroki Shinohara, Jumpei Teramachi, Hirohiko Okamura, Di Yang, Toshihiko Nagata and Tatsuji Haneji : Double stranded RNA-dependent protein kinase is necessary for TNF--induced osteoclast formation in vitro and in vivo., Journal of Cellular Biochemistry, Vol.116, No.9, 1957-1967, 2015.
欧文冊子 ● Hiroki Shinohara, Jumpei Teramachi, Hirohiko Okamura, Di Yang, Toshihiko Nagata and Tatsuji Haneji : Double stranded RNA-dependent protein kinase is necessary for TNF--induced osteoclast formation in vitro and in vivo., Journal of Cellular Biochemistry, Vol.116, No.9, 1957-1967, 2015.

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