『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=296113)

EID=296113EID:296113, Map:0, LastModified:2016年1月13日(水) 15:17:52, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[谷口 寿章], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hyodo Kiwamu (日) (読)
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2.谷口 貴子
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3. (英) Manabe Yuki (日) (読)
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4. (英) Kaido Masanori (日) (読)
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5. (英) Mise Kazuyuki (日) (読)
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6. (英) Sugawara Tatsuya (日) (読)
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7.谷口 寿章
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8. (英) Okuno Tetsuro (日) (読)
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題名 (必須): (英) Phosphatidic Acid produced by phospholipase d promotes RNA replication of a plant RNA virus.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Eukaryotic positive-strand RNA [(+)RNA] viruses are intracellular obligate parasites replicate using the membrane-bound replicase complexes that contain multiple viral and host components. To replicate, (+)RNA viruses exploit host resources and modify host metabolism and membrane organization. Phospholipase D (PLD) is a phosphatidylcholine- and phosphatidylethanolamine-hydrolyzing enzyme that catalyzes the production of phosphatidic acid (PA), a lipid second messenger that modulates diverse intracellular signaling in various organisms. PA is normally present in small amounts (less than 1% of total phospholipids), but rapidly and transiently accumulates in lipid bilayers in response to different environmental cues such as biotic and abiotic stresses in plants. However, the precise functions of PLD and PA remain unknown. Here, we report the roles of PLD and PA in genomic RNA replication of a plant (+)RNA virus, Red clover necrotic mosaic virus (RCNMV). We found that RCNMV RNA replication complexes formed in Nicotiana benthamiana contained PLDα and PLDβ. Gene-silencing and pharmacological inhibition approaches showed that PLDs and PLDs-derived PA are required for viral RNA replication. Consistent with this, exogenous application of PA enhanced viral RNA replication in plant cells and plant-derived cell-free extracts. We also found that a viral auxiliary replication protein bound to PA in vitro, and that the amount of PA increased in RCNMV-infected plant leaves. Together, our findings suggest that RCNMV hijacks host PA-producing enzymes to replicate.  (日)    [継承]
キーワード (推奨):
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誌名 (必須): PLoS Pathogens (Public Library of Science)
(pISSN: 1553-7366, eISSN: 1553-7374)

ISSN (任意): 1553-7374
ISSN: 1553-7366 (pISSN: 1553-7366, eISSN: 1553-7374)
Title: PLoS pathogens
Title(ISO): PLoS Pathog
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 11 [継承]
(必須): 5 [継承]
(必須): e1004909 e1004909 [継承]
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年月日 (必須): 西暦 2015年 5月 28日 (平成 27年 5月 28日) [継承]
URL (任意):
DOI (任意): 10.1371/journal.ppat.1004909    (→Scopusで検索) [継承]
PMID (任意): 26020241    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000355269300048 [継承]
Scopus (任意): 2-s2.0-84930367041 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.ppat.1004909  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) OtherID: PMC4447390  (日)    [継承]

標準的な表示

和文冊子 ● Kiwamu Hyodo, Takako Taniguchi, Yuki Manabe, Masanori Kaido, Kazuyuki Mise, Tatsuya Sugawara, Hisaaki Taniguchi and Tetsuro Okuno : Phosphatidic Acid produced by phospholipase d promotes RNA replication of a plant RNA virus., PLoS Pathogens, Vol.11, No.5, e1004909, 2015.
欧文冊子 ● Kiwamu Hyodo, Takako Taniguchi, Yuki Manabe, Masanori Kaido, Kazuyuki Mise, Tatsuya Sugawara, Hisaaki Taniguchi and Tetsuro Okuno : Phosphatidic Acid produced by phospholipase d promotes RNA replication of a plant RNA virus., PLoS Pathogens, Vol.11, No.5, e1004909, 2015.

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