| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 丸橋 拓海
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Kii Isao (日) (読)
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| 3. | (英) Saito Mitsuru (日) (読)
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| ○貢献度 (任意): |
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| 4. | (英) Kudo Akira (日) (読)
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| ○題名 (必須): | □ | (英) Interaction between periostin and BMP-1 promotes proteolytic activation of lysyl oxidase. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Intra- and intermolecular covalent cross-linking between collagen fibrils, catalyzed by lysyl oxidase (LOX), determines the mechanical properties of connective tissues; however, mechanisms that regulate the collagen cross-linking according to tissue specificity are not well understood. Here we show that periostin, a secretory protein in the dense connective tissues, promotes the activation of LOX. Previous studies showed that periostin null mice exhibit reduced collagen cross-linking in their femurs, periosteum, infarcted myocardium, and tendons. Presently, we showed that active LOX protein, formed by cleavage of its propeptide by bone morphogenetic protein-1 (BMP-1), was decreased in calvarial osteoblast cells derived from periostin null mice. Overexpression of periostin promoted the proteolytic cleavage of the propeptide, which increased the amount of active LOX protein. The results of co-immunoprecipitation and solid phase binding assays revealed that periostin interacted with BMP-1. Furthermore, this interaction probably resulted in enhanced deposition of BMP-1 on the extracellular matrix, suggesting that this enhanced deposition would lead to cleavage of the propeptide of LOX. Thus, we demonstrated that periostin supported BMP-1-mediated proteolytic activation of LOX on the extracellular matrix, which promoted collagen cross-linking. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Bone Morphogenetic Protein 1 (日) (読)
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| 3. | (英) Cell Adhesion Molecules (日) (読)
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| 4. | (英) Cell Line (日) (読)
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| 5. | コラーゲン (collagen)
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| 6. | 細胞外マトリックス (extracellular matrix)
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| 7. | (英) Humans (日) (読)
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| 8. | (英) Mice (日) (読)
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| 9. | ノックアウトマウス (knockout mice)
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| 10. | (英) Osteoblasts (日) (読)
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| 11. | (英) Protein Precursors (日) (読)
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| 12. | (英) Protein-Lysine 6-Oxidase (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
| ○ISSN (任意): | □ | 1083-351X
ISSN: 0021-9258
(pISSN: 0021-9258, eISSN: 1083-351X) Title: The Journal of biological chemistryTitle(ISO): J Biol ChemPublisher: American Society for Biochemistry and Molecular Biology (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 285
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| ○号 (必須): | □ | 17
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| ○頁 (必須): | □ | 13294 13303
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2010年 2月 24日 (平成 22年 2月 24日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1074/jbc.M109.088864 (→Scopusで検索)
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| ○PMID (任意): | □ | 20181949 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-77951235064
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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