| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 山野 範子
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Kimura Tohru (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 3. | (英) Watanabe-Kushima Shoko (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 4. | (英) Shinohara Takashi (日) (読)
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Nakano Toru (日) (読)
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| ○題名 (必須): | □ | (英) Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Specification to primordial germ cells (PGCs) is mediated by mesoderm-induction signals during gastrulation. We found that Akt activation during in vitro mesodermal differentiation of embryonic stem cells (ESCs) generated self-renewing spheres with differentiation states between those of ESCs and PGCs. Essential regulators for PGC specification and their downstream germ cell-specific genes were expressed in the spheres, indicating that the sphere cells had commenced differentiation to the germ lineage. However, the spheres did not proceed to spermatogenesis after transplantation into testes. Sphere cell transfer to the original feeder-free ESC cultures resulted in chaotic differentiation. In contrast, when the spheres were cultured on mouse embryonic fibroblasts or in the presence of ERK-cascade and GSK3 inhibitors, reversion to the ESC-like state was observed. These results indicate that Akt signaling promotes a novel metastable and pluripotent state that is intermediate to those of ESCs and PGCs. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | 細胞分化 (cell differentiation)
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| 3. | (英) Cell Lineage (日) (読)
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| 4. | (英) Cells, Cultured (日) (読)
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| 5. | (英) Embryonic Stem Cells (日) (読)
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| 6. | (英) Enzyme Activation (日) (読)
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| 7. | (英) Epigenesis, Genetic (日) (読)
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| 8. | 遺伝子発現 (gene expression)
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| 9. | (英) Gene Expression Regulation, Developmental (日) (読)
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| 10. | (英) Germ Cells (日) (読)
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| 11. | (英) Mesoderm (日) (読)
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| 12. | (英) Mice (日) (読)
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| 13. | (英) Pluripotent Stem Cells (日) (読)
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| 14. | (英) Proto-Oncogene Proteins c-akt (日) (読)
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| 15. | (英) Spermatogenesis (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 1090-2104
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 392
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 311 316
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2010年 2月 初日 (平成 22年 2月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.bbrc.2010.01.005 (→Scopusで検索)
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| ○PMID (任意): | □ | 20059970 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000275019800012
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| ○Scopus (任意): | □ | 2-s2.0-76349087812
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| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1016/j.bbrc.2010.01.005 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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