| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Kikukawa Hiroshi (日) (読)
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| 2. | 櫻谷 英治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 3. | (英) Nakatani Masato (日) (読)
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| 4. | (英) Ando Akinori (日) (読)
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| 5. | (英) Okuda Tomoyo (日) (読)
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| 6. | 阪本 鷹行 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 7. | (英) Ochiai Misa (日) (読)
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| 8. | (英) Shimizu Sakayu (日) (読)
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| 9. | (英) Ogawa Jun (日) (読)
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| ○題名 (必須): | □ | (英) Gene targeting in the oil-producing fungus Mortierella alpina 1S-4 and construction of a strain producing a valuable polyunsaturated fatty acid. (日)
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| ○要約 (任意): | □ | (英) To develop an efficient gene-targeting system in Mortierella alpina 1S-4, we identified the ku80 gene encoding the Ku80 protein, which is involved in the nonhomologous end-joining pathway in genomic double-strand break (DSB) repair, and constructed ku80 gene-disrupted strains via single-crossover homologous recombination. The ku80 strain from M. alpina 1S-4 showed no negative effects on vegetative growth, formation of spores, and fatty acid productivity, and exhibited high sensitivity to methyl methanesulfonate, which causes DSBs. Dihomo--linolenic acid (DGLA)-producing strains were constructed by disruption of the 5-desaturase gene, encoding a key enzyme of bioconversion of DGLA to ARA, using the ku80 strain as a host strain. The significant improvement of gene-targeting efficiency was not observed by disruption of the ku80 gene, but the construction of DGLA-producing strain by disruption of the 5-desaturase gene was succeeded using the ku80 strain as a host strain. This report describes the first study on the identification and disruption of the ku80 gene in zygomycetes and construction of a DGLA-producing transformant using a gene-targeting system in M. alpina 1S-4. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Current Genetics ([Springer-Verlag])
(pISSN: 0172-8083, eISSN: 1432-0983)
| ○ISSN (任意): | □ | 1432-0983
ISSN: 0172-8083
(pISSN: 0172-8083, eISSN: 1432-0983) Title: Current geneticsTitle(ISO): Curr GenetSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
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| ○巻 (必須): | □ | 61
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 579 589
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| ○年月日 (必須): | □ | 西暦 2015年 3月 18日 (平成 27年 3月 18日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00294-015-0481-2 (→Scopusで検索)
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| ○PMID (任意): | □ | 25782448 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000363043700011
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| ○Scopus (任意): | □ | 2-s2.0-84944513241
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| ○備考 (任意): | 1. | (英) PublicationType: JOURNAL ARTICLE (日)
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