『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=291422EID:291422, Map:0, LastModified:2016年1月21日(木) 13:44:30, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[黒田 暁生], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Ishikawa Kota (日) (読)
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2. (英) Tsunekawa Shin (日) (読)
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3. (英) Ikeniwa Makoto (日) (読)
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4. (英) Izumoto Takako (日) (読)
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5. (英) Iida Atsushi (日) (読)
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6. (英) Ogata Hidetada (日) (読)
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7. (英) Uenishi Eita (日) (読)
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8. (英) Seino Yusuke (日) (読)
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9. (英) Ozaki Nobuaki (日) (読)
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10. (英) Sugimura Yoshihisa (日) (読)
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11. (英) Hamada Yoji (日) (読)
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12.黒田 暁生 ([徳島大学.先端酵素学研究所.基幹研究部門])
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13. (英) Shinjo Keiko (日) (読)
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14. (英) Kondo Yutaka (日) (読)
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15. (英) Oiso Yutaka (日) (読)
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題名 (必須): (英) Long-Term Pancreatic Beta Cell Exposure to High Levels of Glucose but Not Palmitate Induces DNA Methylation within the Insulin Gene Promoter and Represses Transcriptional Activity.  (日)    [継承]
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要約 (任意): (英) Recent studies have implicated epigenetics in the pathophysiology of diabetes. Furthermore, DNA methylation, which irreversibly deactivates gene transcription, of the insulin promoter, particularly the cAMP response element, is increased in diabetes patients. However, the underlying mechanism remains unclear. We aimed to investigate insulin promoter DNA methylation in an over-nutrition state. INS-1 cells, the rat pancreatic beta cell line, were cultured under normal-culture-glucose (11.2 mmol/l) or experimental-high-glucose (22.4 mmol/l) conditions for 14 days, with or without 0.4 mmol/l palmitate. DNA methylation of the rat insulin 1 gene (Ins1) promoter was investigated using bisulfite sequencing and pyrosequencing analysis. Experimental-high-glucose conditions significantly suppressed insulin mRNA and increased DNA methylation at all five CpG sites within the Ins1 promoter, including the cAMP response element, in a time-dependent and glucose concentration-dependent manner. DNA methylation under experimental-high-glucose conditions was unique to the Ins1 promoter; however, palmitate did not affect DNA methylation. Artificial methylation of Ins1 promoter significantly suppressed promoter-driven luciferase activity, and a DNA methylation inhibitor significantly improved insulin mRNA suppression by experimental-high-glucose conditions. Experimental-high-glucose conditions significantly increased DNA methyltransferase activity and decreased ten-eleven-translocation methylcytosine dioxygenase activity. Oxidative stress and endoplasmic reticulum stress did not affect DNA methylation of the Ins1 promoter. High glucose but not palmitate increased ectopic triacylglycerol accumulation parallel to DNA methylation. Metformin upregulated insulin gene expression and suppressed DNA methylation and ectopic triacylglycerol accumulation. Finally, DNA methylation of the Ins1 promoter increased in isolated islets from Zucker diabetic fatty rats. This study helps to clarify the effect of an over-nutrition state on DNA methylation of the Ins1 promoter in pancreatic beta cells. It provides new insights into the irreversible pathophysiology of diabetes.  (日)    [継承]
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誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 10 [継承]
(必須): 2 [継承]
(必須): e0115350 e0115350 [継承]
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年月日 (必須): 西暦 2015年 2月 6日 (平成 27年 2月 6日) [継承]
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DOI (任意): 10.1371/journal.pone.0115350    (→Scopusで検索) [継承]
PMID (任意): 25658116    (→Scopusで検索) [継承]
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WOS (任意): 000349444900016 [継承]
Scopus (任意): 2-s2.0-84922714378 [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Kota Ishikawa, Shin Tsunekawa, Makoto Ikeniwa, Takako Izumoto, Atsushi Iida, Hidetada Ogata, Eita Uenishi, Yusuke Seino, Nobuaki Ozaki, Yoshihisa Sugimura, Yoji Hamada, Akio Kuroda, Keiko Shinjo, Yutaka Kondo and Yutaka Oiso : Long-Term Pancreatic Beta Cell Exposure to High Levels of Glucose but Not Palmitate Induces DNA Methylation within the Insulin Gene Promoter and Represses Transcriptional Activity., PLoS ONE, Vol.10, No.2, e0115350, 2015.
欧文冊子 ● Kota Ishikawa, Shin Tsunekawa, Makoto Ikeniwa, Takako Izumoto, Atsushi Iida, Hidetada Ogata, Eita Uenishi, Yusuke Seino, Nobuaki Ozaki, Yoshihisa Sugimura, Yoji Hamada, Akio Kuroda, Keiko Shinjo, Yutaka Kondo and Yutaka Oiso : Long-Term Pancreatic Beta Cell Exposure to High Levels of Glucose but Not Palmitate Induces DNA Methylation within the Insulin Gene Promoter and Represses Transcriptional Activity., PLoS ONE, Vol.10, No.2, e0115350, 2015.

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