『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hatakeyama Tomomitsu (日) (読)
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2. (英) Ichise Ayaka (日) (読)
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3. (英) Yonekura Tomokazu (日) (読)
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4. (英) Unno Hideaki (日) (読)
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5.郷田 秀一郎 ([長崎大学])
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6.中川 秀幸
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題名 (必須): (英) cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom  (日)    [継承]
副題 (任意):
要約 (任意): (英) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contain several biologically active proteins. Among these, a galactose-binding lectin SUL-I isolated from the venom in the large globiferous pedicellariae shows several activities such as mitogenic, chemotactic, and cytotoxic activities through binding to the carbohydrate chains on the cells. We cloned cDNA encoding SUL-I by reverse transcription-PCR using the degenerate primers designed on the basis of the N-terminal amino acid sequence of the protein and expressed the recombinant SUL-I (rSUL-I) in Escherichia coli cells. The SUL-I gene contains an open reading frame of 927 nucleotides corresponding to 308 amino acid residues, including 24 residues of a putative signal sequence. The mature protein with 284 residues is composed of three homologous regions, each showing similarity with the carbohydrate-recognition domains of the rhamnose-binding lectins, which have been mostly found in fish eggs. While rSUL-I exhibited binding activity for several galactose-related sugars, the highest affinity was found for l-rhamnose among carbohydrates tested, confirming that SUL-I is a rhamnose-binding lectin. rSUL-I also showed hemagglutinating activity toward rabbit erythrocytes, indicating the existence of more than one carbohydrate-binding site to cross-link the carbohydrate chains on the cell surface, which may be closely related to its biological activities.  (日)    [継承]
キーワード (推奨): 1. (英) sea urchin venom (日) (読) [継承]
2. (英) Toxopneustes pileolus (日) (読) [継承]
3. (英) pedicellaria (日) (読) [継承]
4. (英) rhmnose-binding lectin (日) (読) [継承]
5. (英) cDNA cloning (日) (読) [継承]
発行所 (推奨): Elsevier (->組織[Elsevier Science]) [継承]
誌名 (必須): Toxicon (International Society on Toxinology)
(pISSN: 0041-0101, eISSN: 1879-3150)

ISSN (任意): 1879-3150
ISSN: 0041-0101 (pISSN: 0041-0101, eISSN: 1879-3150)
Title: Toxicon : official journal of the International Society on Toxinology
Title(ISO): Toxicon
Publisher: Elsevier Ltd.
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年月日 (必須): 西暦 2015年 1月 24日 (平成 27年 1月 24日) [継承]
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DOI (任意): 10.1016/j.toxicon.2014.11.236    (→Scopusで検索) [継承]
PMID (任意): 25475394    (→Scopusで検索) [継承]
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WOS (任意): 000349198400002 [継承]
Scopus (任意): 2-s2.0-84918582853 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1016/j.toxicon.2014.11.236  (日)    [継承]
2.(英) Article.ELocationID: S0041-0101(14)00607-2  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) KeywordList.Keyword: Carbohydrate-binding  (日)    [継承]
6.(英) KeywordList.Keyword: Lectin  (日)    [継承]
7.(英) KeywordList.Keyword: Rhamnose  (日)    [継承]
8.(英) KeywordList.Keyword: Sea urchin  (日)    [継承]
9.(英) KeywordList.Keyword: Toxopneustes pileolus  (日)    [継承]
10.(英) KeywordList.Keyword: cDNA cloning  (日)    [継承]

標準的な表示

和文冊子 ● Tomomitsu Hatakeyama, Ayaka Ichise, Tomokazu Yonekura, Hideaki Unno, Shuichiro Goda and Hideyuki Nakagawa : cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom, Toxicon, Vol.94, No.1, 8-15, 2015.
欧文冊子 ● Tomomitsu Hatakeyama, Ayaka Ichise, Tomokazu Yonekura, Hideaki Unno, Shuichiro Goda and Hideyuki Nakagawa : cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom, Toxicon, Vol.94, No.1, 8-15, 2015.

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