『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=291057)

EID=291057EID:291057, Map:0, LastModified:2015年3月3日(火) 21:54:55, Operator:[山田 久嗣], Avail:TRUE, Censor:0, Owner:[山田 久嗣], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.山田 久嗣 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Tanabe Kazuhito (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Nishimoto Sei-ichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Photocurrent response after enzymatic treatment of DNA duplexes immobilized on gold electrodes: electrochemical discrimination of 5-methylcytosine modification in DNA.  (日)    [継承]
副題 (任意):
要約 (任意): (英) We demonstrate a photoelectrochemical approach to the detection of the methylation status of cytosine bases in DNA. We prepared anthraquinone (AQ) photosensitizer-tethered oligodeoxynucleotide (ODN) duplexes bearing 5-methylcytosine (mC) or the corresponding cytosine (C) at a restriction site of the ODN strand immobilized on gold electrodes, and measured their photocurrent responses arising from hole transport after enzymatic digestion. Treatment with HapII or HhaI of the duplexes bearing normal C led to strand cleavage, and the photosensitizer unit was eliminated from the ODN strand immobilized on the gold electrode, exclusively reducing the photocurrent density. With a similar treatment, the duplexes bearing mC showed higher photocurrent responses arising from hole transport through the duplex. This significant difference in the photocurrent response between mC and normal C residues in DNA on the gold electrodes is potentially applicable to the detection of mC modification in DNA.  (日)    [継承]
キーワード (推奨): 1. (英) 5-Methylcytosine (日) (読) [継承]
2. (英) Anthraquinones (日) (読) [継承]
3. (英) Cytosine (日) (読) [継承]
4. (英) DNA (日) (読) [継承]
5. (英) DNA Methylation (日) (読) [継承]
6. (英) Deoxyribonuclease HpaII (日) (読) [継承]
7. (英) Deoxyribonucleases, Type II Site-Specific (日) (読) [継承]
8. (英) Electrochemistry (日) (読) [継承]
9. (英) Electrodes (日) (読) [継承]
10. (英) Electroretinography (日) (読) [継承]
11. (英) Gold (日) (読) [継承]
12. (英) Molecular Conformation (日) (読) [継承]
13. (英) Oligodeoxyribonucleotides (日) (読) [継承]
14. (英) Photochemistry (日) (読) [継承]
15. (英) Photosensitizing Agents (日) (読) [継承]
16. (英) Time Factors (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Organic & Biomolecular Chemistry ([英国王立化学会])
(pISSN: 1477-0520, eISSN: 1477-0539)

ISSN (任意): 1477-0520
ISSN: 1477-0520 (pISSN: 1477-0520, eISSN: 1477-0539)
Title: Organic & biomolecular chemistry
Title(ISO): Org Biomol Chem
Publisher: Royal Society of Chemistry
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 6 [継承]
(必須): 2 [継承]
(必須): 272 277 [継承]
都市 (任意):
年月日 (必須): 西暦 2007年 12月 3日 (平成 19年 12月 3日) [継承]
URL (任意):
DOI (任意): 10.1039/b715260d    (→Scopusで検索) [継承]
PMID (任意): 18174996    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hisatsugu Yamada, Kazuhito Tanabe and Sei-ichi Nishimoto : Photocurrent response after enzymatic treatment of DNA duplexes immobilized on gold electrodes: electrochemical discrimination of 5-methylcytosine modification in DNA., Organic & Biomolecular Chemistry, Vol.6, No.2, 272-277, 2007.
欧文冊子 ● Hisatsugu Yamada, Kazuhito Tanabe and Sei-ichi Nishimoto : Photocurrent response after enzymatic treatment of DNA duplexes immobilized on gold electrodes: electrochemical discrimination of 5-methylcytosine modification in DNA., Organic & Biomolecular Chemistry, Vol.6, No.2, 272-277, 2007.

関連情報

Number of session users = 0, LA = 0.64, Max(EID) = 382692, Max(EOID) = 1022065.