『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=290285)

EID=290285EID:290285, Map:0, LastModified:2015年7月3日(金) 16:18:40, Operator:[谷口 寿章], Avail:TRUE, Censor:0, Owner:[谷口 寿章], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kaido Masanori (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Abe Kazutomo (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Mine Akira (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Hyodo Kiwamu (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.谷口 貴子
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.谷口 寿章
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Mise Kazuyuki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Okuno Tetsuro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) GAPDH--a recruits a plant virus movement protein to cortical virus replication complexes to facilitate viral cell-to-cell movement.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The formation of virus movement protein (MP)-containing punctate structures on the cortical endoplasmic reticulum is required for efficient intercellular movement of Red clover necrotic mosaic virus (RCNMV), a bipartite positive-strand RNA plant virus. We found that these cortical punctate structures constitute a viral replication complex (VRC) in addition to the previously reported aggregate structures that formed adjacent to the nucleus. We identified host proteins that interacted with RCNMV MP in virus-infected Nicotiana benthamiana leaves using a tandem affinity purification method followed by mass spectrometry. One of these host proteins was glyceraldehyde 3-phosphate dehydrogenase-A (NbGAPDH-A), which is a component of the Calvin-Benson cycle in chloroplasts. Virus-induced gene silencing of NbGAPDH-A reduced RCNMV multiplication in the inoculated leaves, but not in the single cells, thereby suggesting that GAPDH-A plays a positive role in cell-to-cell movement of RCNMV. The fusion protein of NbGAPDH-A and green fluorescent protein localized exclusively to the chloroplasts. In the presence of RCNMV RNA1, however, the protein localized to the cortical VRC as well as the chloroplasts. Bimolecular fluorescence complementation assay and GST pulldown assay confirmed in vivo and in vitro interactions, respectively, between the MP and NbGAPDH-A. Furthermore, gene silencing of NbGAPDH-A inhibited MP localization to the cortical VRC. We discuss the possible roles of NbGAPDH-A in the RCNMV movement process.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): PLoS Pathogens (Public Library of Science)
(pISSN: 1553-7366, eISSN: 1553-7374)

ISSN (任意): 1553-7374
ISSN: 1553-7366 (pISSN: 1553-7366, eISSN: 1553-7374)
Title: PLoS pathogens
Title(ISO): PLoS Pathog
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 10 [継承]
(必須): 11 [継承]
(必須): e1004505 e1004505 [継承]
都市 (任意):
年月日 (必須): 西暦 2014年 11月 20日 (平成 26年 11月 20日) [継承]
URL (任意):
DOI (任意): 10.1371/journal.ppat.1004505    (→Scopusで検索) [継承]
PMID (任意): 25411849    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.ppat.1004505  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC4239097  (日)    [継承]

標準的な表示

和文冊子 ● Masanori Kaido, Kazutomo Abe, Akira Mine, Kiwamu Hyodo, Takako Taniguchi, Hisaaki Taniguchi, Kazuyuki Mise and Tetsuro Okuno : GAPDH--a recruits a plant virus movement protein to cortical virus replication complexes to facilitate viral cell-to-cell movement., PLoS Pathogens, Vol.10, No.11, e1004505, 2014.
欧文冊子 ● Masanori Kaido, Kazutomo Abe, Akira Mine, Kiwamu Hyodo, Takako Taniguchi, Hisaaki Taniguchi, Kazuyuki Mise and Tetsuro Okuno : GAPDH--a recruits a plant virus movement protein to cortical virus replication complexes to facilitate viral cell-to-cell movement., PLoS Pathogens, Vol.10, No.11, e1004505, 2014.

関連情報

Number of session users = 3, LA = 0.30, Max(EID) = 374181, Max(EOID) = 1002074.