『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=290230EID:290230, Map:0, LastModified:2015年9月8日(火) 20:40:51, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[川上 竜巳], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
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貢献度 (任意):
学籍番号 (推奨):
[継承]
2.櫻庭 春彦
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貢献度 (任意):
学籍番号 (推奨):
[継承]
3.大島 敏久 ([九州大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis  (日)    [継承]
副題 (任意):
要約 (任意): (英) We previously found a very large NAD-dependent glutamate dehydrogenase with approximately 170 kDa subunit from Janthinobacterium lividum (Jl-GDH) and predicted that GDH reaction occurred in the central domain of the subunit. To gain further insights into the role of the central domain, several single point mutations were introduced. The enzyme activity was completely lost in all single mutants of R784A, K810A, K820A, D885A, and S1142A. Because, in sequence alignment analysis, these residues corresponded to the residues responsible for glutamate binding in well-known small GDH with approximately 50 kDa subunit, very large GDH and well-known small GDH may share the same catalytic mechanism. In addition, we demonstrated that C1141, one of the three cysteine residues in the central domain, was responsible for the inhibition of enzyme activity by HgCl2, and HgCl2 functioned as an activating compound for a C1141T mutant. At low concentrations, moreover, HgCl2 was found to function as an activating compound for a wild-type Jl-GDH. This suggests that the mechanism for the activation is entirely different from that for the inhibition.  (日)    [継承]
キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Bacterial Proteins (日) (読) [継承]
3. (英) Binding Sites (日) (読) [継承]
4. (英) Biocatalysis (日) (読) [継承]
5. (英) Burkholderiaceae (日) (読) [継承]
6. (英) Cysteine (日) (読) [継承]
7. (英) Enzyme Assays (日) (読) [継承]
8. (英) Gene Expression (日) (読) [継承]
9. (英) Glutamate Dehydrogenase (日) (読) [継承]
10. (英) Glutamic Acid (日) (読) [継承]
11. (英) Kinetics (日) (読) [継承]
12. (英) Mercuric Chloride (日) (読) [継承]
13. (英) Molecular Sequence Data (日) (読) [継承]
14. (英) Mutagenesis, Site-Directed (日) (読) [継承]
15. (英) Mutation (日) (読) [継承]
16. (英) Protein Binding (日) (読) [継承]
17. (英) Protein Structure, Tertiary (日) (読) [継承]
18. (英) Protein Subunits (日) (読) [継承]
19. (英) Sequence Homology, Amino Acid (日) (読) [継承]
20. (英) Structure-Activity Relationship (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Bioscience, Biotechnology, and Biochemistry ([社団法人 日本農芸化学会])
(pISSN: 0916-8451, eISSN: 1347-6947)

ISSN (任意): 1347-6947
ISSN: 0916-8451 (pISSN: 0916-8451, eISSN: 1347-6947)
Title: Bioscience, biotechnology, and biochemistry
Title(ISO): Biosci Biotechnol Biochem
Supplier: 社団法人日本農芸化学会
Publisher: Taylor & Francis
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 78 [継承]
(必須): 12 [継承]
(必須): 2045 2050 [継承]
都市 (任意):
年月日 (必須): 西暦 2014年 12月 初日 (平成 26年 12月 初日) [継承]
URL (任意):
DOI (任意): 10.1080/09168451.2014.946394    (→Scopusで検索) [継承]
PMID (任意): 25126984    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000345504400010 [継承]
Scopus (任意): 2-s2.0-84924529811 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1080/09168451.2014.946394  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) KeywordList.Keyword: Janthinobacterium lividum  (日)    [継承]
4.(英) KeywordList.Keyword: activation by Hg2+  (日)    [継承]
5.(英) KeywordList.Keyword: glutamate-binding residues  (日)    [継承]
6.(英) KeywordList.Keyword: inhibition by Hg2+  (日)    [継承]
7.(英) KeywordList.Keyword: very large glutamate dehydrogenase  (日)    [継承]

標準的な表示

和文冊子 ● Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima : Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis, Bioscience, Biotechnology, and Biochemistry, Vol.78, No.12, 2045-2050, 2014.
欧文冊子 ● Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima : Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis, Bioscience, Biotechnology, and Biochemistry, Vol.78, No.12, 2045-2050, 2014.

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