『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Yamazoe Mitsuyoshi (日) (読)
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2.足立 隼
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3. (英) Kanaya Shigehiko (日) (読)
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4. (英) Ohsumi Katsufumi (日) (読)
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5. (英) Hiraga Sota (日) (読)
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題名 (必須): (英) Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli.  (日)    [継承]
副題 (任意):
要約 (任意): (英) To demonstrate that sequestration A (SeqA) protein binds preferentially to hemimethylated GATC sequences at replication forks and forms clusters in Escherichia coli growing cells, we analysed, by the chromatin immunoprecipitation (ChIP) assay using anti-SeqA antibody, a synchronized culture of a temperature-sensitive dnaC mutant strain in which only one round of chromosomal DNA replication was synchronously initiated. After synchronized initiation of chromosome replication, the replication origin oriC was first detected by the ChIP assay, and other six chromosomal regions having multiple GATC sequences were sequentially detected according to bidirectional replication of the chromosome. In contrast, DNA regions lacking the GATC sequence were not detected by the ChIP assay. These results indicate that SeqA binds hemimethylated nascent DNA segments according to the proceeding of replication forks in the chromosome, and SeqA releases from the DNA segments when fully methylated. Immunofluorescence microscopy reveals that a single SeqA focus containing paired replication apparatuses appears at the middle of the cell immediately after initiation of chromosome replication and the focus is subsequently separated into two foci that migrate to 1/4 and 3/4 cellular positions, when replication forks proceed bidirectionally an approximately one-fourth distance from the replication origin towards the terminus. This supports the translocating replication apparatuses model.  (日)    [継承]
キーワード (推奨): 1. (英) Bacterial Outer Membrane Proteins (日) (読) [継承]
2. (英) Chromatin Immunoprecipitation (日) (読) [継承]
3. (英) DNA Replication (日) (読) [継承]
4. (英) DNA, Bacterial (日) (読) [継承]
5. (英) DNA-Binding Proteins (日) (読) [継承]
6.大腸菌 (Escherichia coli) [継承]
7. (英) Escherichia coli Proteins (日) (読) [継承]
8. (英) Kinetics (日) (読) [継承]
9. (英) Microscopy, Fluorescence (日) (読) [継承]
10. (英) Protein Binding (日) (読) [継承]
11. (英) Staining and Labeling (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Molecular Microbiology ([Blackwell Publishing Ltd])
(pISSN: 0950-382X, eISSN: 1365-2958)

ISSN (任意): 0950-382X
ISSN: 0950-382X (pISSN: 0950-382X, eISSN: 1365-2958)
Title: Molecular microbiology
Title(ISO): Mol Microbiol
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2005年 1月 初日 (平成 17年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1365-2958.2004.04389.x    (→Scopusで検索) [継承]
PMID (任意): 15612935    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000225949300025 [継承]
Scopus (任意): 2-s2.0-12344308528 [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mitsuyoshi Yamazoe, Shun Adachi, Shigehiko Kanaya, Katsufumi Ohsumi and Sota Hiraga : Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli., Molecular Microbiology, Vol.55, No.1, 289-298, 2005.
欧文冊子 ● Mitsuyoshi Yamazoe, Shun Adachi, Shigehiko Kanaya, Katsufumi Ohsumi and Sota Hiraga : Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli., Molecular Microbiology, Vol.55, No.1, 289-298, 2005.

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