『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨):
著者 (必須): 1.足立 隼
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2. (英) Kohiyama Masamichi (日) (読)
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3. (英) Onogi Toshinari (日) (読)
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4. (英) Hiraga Sota (日) (読)
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題名 (必須): (英) Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli.  (日)    [継承]
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要約 (任意): (英) To examine the subcellular localization of the replication machinery in Escherichia coli, we have developed an immunofluorescence method that allows us to determine the subcellular location of newly synthesized DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU). Using this technique, we have analyzed growing cells. In wild-type cells that showed a single BrdU fluorescence signal, the focus was located in the middle of the cell; in cells with two signals, the foci were localized at positions equivalent to 1/4 and 3/4 of the cell length. The formation of BrdU foci was dependent upon ongoing chromosomal replication. A mutant lacking MukB, which is required for proper partitioning of sister chromosomes, failed to maintain the ordered localization of BrdU foci: (1) a single BrdU focus tended to be localized at a pole-proximal region of the nucleoid, and (2) a focus was often found to consist of two replicating chromosomes. Thus, the positioning of replication forks is affected by the disruption of the mukB gene.  (日)    [継承]
キーワード (推奨): 1. (英) Bromodeoxyuridine (日) (読) [継承]
2. (英) Chromosomal Proteins, Non-Histone (日) (読) [継承]
3.DNA (DNA) [継承]
4. (英) DNA Replication (日) (読) [継承]
5.大腸菌 (Escherichia coli) [継承]
6. (英) Escherichia coli Proteins (日) (読) [継承]
7. (英) Flow Cytometry (日) (読) [継承]
8. (英) Fluorescent Antibody Technique, Indirect (日) (読) [継承]
9.免疫組織化学 (immunohistochemistry) [継承]
発行所 (推奨):
誌名 (必須): Molecular Genetics and Genomics : MGG (Springer Berlin Heidelberg)
(pISSN: 1617-4615, eISSN: 1617-4623)

ISSN (任意): 1617-4615
ISSN: 1617-4615 (pISSN: 1617-4615, eISSN: 1617-4623)
Title: Molecular genetics and genomics : MGG
Title(ISO): Mol Genet Genomics
Publisher: Springer Berlin Heidelberg
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 274 [継承]
(必須): 3 [継承]
(必須): 264 271 [継承]
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年月日 (必須): 西暦 2005年 10月 20日 (平成 17年 10月 20日) [継承]
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DOI (任意): 10.1007/s00438-005-0023-6    (→Scopusで検索) [継承]
PMID (任意): 16133165    (→Scopusで検索) [継承]
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WOS (任意): 000232722000007 [継承]
Scopus (任意): 2-s2.0-26944479228 [継承]
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備考 (任意): 1.(英) PublicationType: Comparative Study  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]
3.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Shun Adachi, Masamichi Kohiyama, Toshinari Onogi and Sota Hiraga : Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli., Molecular Genetics and Genomics : MGG, Vol.274, No.3, 264-271, 2005.
欧文冊子 ● Shun Adachi, Masamichi Kohiyama, Toshinari Onogi and Sota Hiraga : Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli., Molecular Genetics and Genomics : MGG, Vol.274, No.3, 264-271, 2005.

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