| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 足立 隼
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| 2. | (英) Kohiyama Masamichi (日) (読)
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| 3. | (英) Onogi Toshinari (日) (読)
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| 4. | (英) Hiraga Sota (日) (読)
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| ○題名 (必須): | □ | (英) Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli. (日)
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| ○要約 (任意): | □ | (英) To examine the subcellular localization of the replication machinery in Escherichia coli, we have developed an immunofluorescence method that allows us to determine the subcellular location of newly synthesized DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU). Using this technique, we have analyzed growing cells. In wild-type cells that showed a single BrdU fluorescence signal, the focus was located in the middle of the cell; in cells with two signals, the foci were localized at positions equivalent to 1/4 and 3/4 of the cell length. The formation of BrdU foci was dependent upon ongoing chromosomal replication. A mutant lacking MukB, which is required for proper partitioning of sister chromosomes, failed to maintain the ordered localization of BrdU foci: (1) a single BrdU focus tended to be localized at a pole-proximal region of the nucleoid, and (2) a focus was often found to consist of two replicating chromosomes. Thus, the positioning of replication forks is affected by the disruption of the mukB gene. (日)
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| ○キーワード (推奨): | 1. | (英) Bromodeoxyuridine (日) (読)
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| 2. | (英) Chromosomal Proteins, Non-Histone (日) (読)
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| 3. | DNA (DNA)
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| 4. | (英) DNA Replication (日) (読)
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| 5. | 大腸菌 (Escherichia coli)
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| 6. | (英) Escherichia coli Proteins (日) (読)
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| 7. | (英) Flow Cytometry (日) (読)
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| 8. | (英) Fluorescent Antibody Technique, Indirect (日) (読)
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| 9. | 免疫組織化学 (immunohistochemistry)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Molecular Genetics and Genomics : MGG (Springer Berlin Heidelberg)
(pISSN: 1617-4615, eISSN: 1617-4623)
| ○ISSN (任意): | □ | 1617-4615
ISSN: 1617-4615
(pISSN: 1617-4615, eISSN: 1617-4623) Title: Molecular genetics and genomics : MGGTitle(ISO): Mol Genet GenomicsPublisher: Springer (NLM Catalog)
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| ○巻 (必須): | □ | 274
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 264 271
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| ○年月日 (必須): | □ | 西暦 2005年 10月 20日 (平成 17年 10月 20日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00438-005-0023-6 (→Scopusで検索)
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| ○PMID (任意): | □ | 16133165 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000232722000007
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| ○Scopus (任意): | □ | 2-s2.0-26944479228
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| ○備考 (任意): | 1. | (英) PublicationType: Comparative Study (日)
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| 2. | (英) PublicationType: Journal Article (日)
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| 3. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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