『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.足立 隼
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Fukushima Toru (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
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3. (英) Hiraga Sota (日) (読)
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題名 (必須): (英) Dynamic events of sister chromosomes in the cell cycle of Escherichia coli.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Various events involving partitioning of sister chromosomes were precisely analyzed in asynchronously growing Escherichia coli cells in various conditions. To examine the cohesion between sister chromosomes, we analyzed living cells growing under various conditions for the number of the replication origin (oriC) copies by flow cytometry and the foci of oriC by fluorescence microscopy. The average number of the oriC foci per cell was significantly smaller than the number of oriC copies per cell with few exceptions, suggesting cohesion of oriC sister copies. Cohesion phenomenon of oriC sister copies was also observed in a mukB null mutant cells under some growth conditions. Sister copies of the terminal region (ter) were also found to be cohesive. Immunofluorescence microscopy for nascent DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU) indicated that paired replication forks acting on bidirectional replication were able to migrate toward opposite directions during ongoing replication in poor medium; however, some of them were closely associated in rich media. Analysis of the foci of MukB-GFP indicates that the number of MukB foci was always larger than the number of replication forks. The number of MukB-GFP foci increased together with cell length. The sequence of these chromosomal events in the growing cells has been depicted.  (日)    [継承]
キーワード (推奨): 1. (英) Bacterial Outer Membrane Proteins (日) (読) [継承]
2. (英) Bromodeoxyuridine (日) (読) [継承]
3.細胞周期 (cell cycle) [継承]
4. (英) Chromosomal Proteins, Non-Histone (日) (読) [継承]
5. (英) Chromosomes, Bacterial (日) (読) [継承]
6. (英) DNA, Bacterial (日) (読) [継承]
7. (英) DNA-Binding Proteins (日) (読) [継承]
8.大腸菌 (Escherichia coli) [継承]
9. (英) Escherichia coli Proteins (日) (読) [継承]
10. (英) Flow Cytometry (日) (読) [継承]
11. (英) Gene Dosage (日) (読) [継承]
12. (英) Genes, Bacterial (日) (読) [継承]
13. (英) Green Fluorescent Proteins (日) (読) [継承]
14. (英) In Situ Hybridization, Fluorescence (日) (読) [継承]
15. (英) Microscopy, Fluorescence (日) (読) [継承]
16. (英) Mutation (日) (読) [継承]
17. (英) Origin Recognition Complex (日) (読) [継承]
18. (英) Recombinant Fusion Proteins (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Genes to Cells ([日本分子生物学会])
(pISSN: 1356-9597, eISSN: 1365-2443)

ISSN (任意): 1365-2443
ISSN: 1356-9597 (pISSN: 1356-9597, eISSN: 1365-2443)
Title: Genes to cells : devoted to molecular & cellular mechanisms
Title(ISO): Genes Cells
Supplier: Molecular Biology Society of Japan
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 2 [継承]
(必須): 181 197 [継承]
都市 (任意):
年月日 (必須): 西暦 2008年 2月 初日 (平成 20年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1365-2443.2007.01157.x    (→Scopusで検索) [継承]
PMID (任意): 18233960    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000252774800007 [継承]
Scopus (任意): 2-s2.0-38849135690 [継承]
評価値 (任意):
被引用数 (任意):
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Shun Adachi, Toru Fukushima and Sota Hiraga : Dynamic events of sister chromosomes in the cell cycle of Escherichia coli., Genes to Cells, Vol.13, No.2, 181-197, 2008.
欧文冊子 ● Shun Adachi, Toru Fukushima and Sota Hiraga : Dynamic events of sister chromosomes in the cell cycle of Escherichia coli., Genes to Cells, Vol.13, No.2, 181-197, 2008.

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