『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=289422EID:289422, Map:0, LastModified:2015年7月4日(土) 21:21:39, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[宮本 洋二], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Kinouchi Makoto (日) (読)
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2.内田 大亮
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3.栗林 伸行
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4.玉谷 哲也
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5.永井 宏和
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6.宮本 洋二 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.口腔外科学])
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題名 (必須): (英) Involvement of miR-518c-5p to Growth and Metastasis in Oral Cancer  (日)    [継承]
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要約 (任意): (英) We have previously demonstrated that a stromal cell-derived factor-1 (SDF-1; CXCL12)/CXCR4 system is involved in the establishment of metastasis in oral cancer. Recently, small non coding RNAs, microRNAs (miRNAs) have been shown to be involved in the metastatic process of several types of cancers. However, the miRNAs that contribute to metastases induced by the SDF-1/CXCR4 system in oral cancer are largely unknown. In this study, we examined the metastasis-related miRNAs induced by the SDF-1/CXCR4 system using B88-SDF-1 oral cancer cells, which exhibit functional CXCR4 and distant metastatic potential in vivo. Through miRNA microarray analysis, we identified the upregulation of miR-518c-5p in B88-SDF-1 cells, and confirmed the induction by real-time PCR analysis. Although an LNA-based miR-518c-5p inhibitor did not affect cell growth of B88-SDF-1 cells, it did significantly inhibit the migration of the cells. Next, we transfected a miR-518c expression vector into parental B88 cells and CAL27 oral cancer cells and isolated stable transfectants, B88-518c and CAL27-518c cells, respectively. The anchorage-dependent and -independent growth of miR-518c transfectants was significantly enhanced compared with the growth of mock cells. Moreover, we detected the enhanced migration of these cells. The LNA-based miR-518c-5p inhibitor significantly impaired the enhanced cell growth and migration of miR-518c transfectants, indicating that these phenomena were mainly dependent on the expression of miR-518c-5p. Next, we examined the function of miR-518c-5p in vivo. miR-518c transfectants or mock transfectants were inoculated into the masseter muscle or the blood vessels of nude mice. Tumor volume, lymph nodes metastasis, and lung metastasis were significantly increased in the mice inoculated with the miR-518c transfectants. These results indicated that miR-518c-5p regulates the growth and metastasis of oral cancer as a downstream target of the SDF-1/CXCR4 system.  (日)    [継承]
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誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS ONE
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): e115936 e115936 [継承]
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年月日 (必須): 西暦 2014年 12月 23日 (平成 26年 12月 23日) [継承]
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DOI (任意): 10.1371/journal.pone.0115936    (→Scopusで検索) [継承]
PMID (任意): 25536052    (→Scopusで検索) [継承]
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WOS (任意): 000348563300089 [継承]
Scopus (任意): 2-s2.0-84919809244 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.pone.0115936  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC4275267  (日)    [継承]

標準的な表示

和文冊子 ● Makoto Kinouchi, Daisuke Uchida, Nobuyuki Kuribayashi, Tetsuya Tamatani, Hirokazu Nagai and Youji Miyamoto : Involvement of miR-518c-5p to Growth and Metastasis in Oral Cancer, PLoS ONE, Vol.9, No.12, e115936, 2014.
欧文冊子 ● Makoto Kinouchi, Daisuke Uchida, Nobuyuki Kuribayashi, Tetsuya Tamatani, Hirokazu Nagai and Youji Miyamoto : Involvement of miR-518c-5p to Growth and Metastasis in Oral Cancer, PLoS ONE, Vol.9, No.12, e115936, 2014.

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