| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Kataoka Michihiko (日) (読)
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| 2. | (英) Ishige Takeru (日) (読)
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| 3. | (英) Urano Nobuyuki (日) (読)
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| 4. | (英) Nakamura Yasushi (日) (読)
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| 5. | 櫻谷 英治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 6. | (英) Fukui Satoko (日) (読)
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| 7. | (英) Kita Shinji (日) (読)
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| 8. | (英) Sakamoto Keiji (日) (読)
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| 9. | (英) Shimizu Sakayu (日) (読)
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| ○題名 (必須): | □ | (英) Cloning and expression of the L-1-amino-2-propanol dehydrogenase gene from Rhodococcus erythropolis, and its application to double chiral compound production. (日)
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| ○要約 (任意): | □ | (英) The gene encoding NADP(+)-dependent L-1-amino-2-propanol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 was cloned and sequenced. A 780-bp nucleotide fragment was confirmed to be the gene encoding AADH by agreement of the N-terminal and internal amino acid sequences of the purified AADH. The gene (aadh) codes a total of 259 amino acid residues, and the deduced amino acid sequence shows similarity to several short-chain dehydrogenase/reductase family proteins. An expression vector, pKKAADH, which contains the full length aadh was constructed. Escherichia coli cells possessing pKKAADH exhibited a 10.4-fold increase in specific activity as to catalysis of the reduction of (S)-1-phenyl-2-methylaminopropan-1-one (MAK), as compared with that of R. erythropolis MAK154 induced by 1-amino-2-propanol (1 mg/ml). Coexpression of aadh with a cofactor regeneration enzyme (glucose dehydrogenase) gene was also performed, and a system for sufficient production of d-pseudoephedrine from racemic MAK was constructed. (日)
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| ○キーワード (推奨): | 1. | (英) Adamantane (日) (読)
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| 2. | (英) Alcohol Oxidoreductases (日) (読)
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| 3. | (英) Bacterial Proteins (日) (読)
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| 4. | (英) Cloning, Molecular (日) (読)
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| 5. | (英) Escherichia coli (日) (読)
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| 6. | (英) Gene Expression (日) (読)
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| 7. | (英) Glucose 1-Dehydrogenase (日) (読)
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| 8. | (英) Molecular Sequence Data (日) (読)
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| 9. | (英) Rhodococcus (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Applied Microbiology and Biotechnology ([Springer-Verlag])
(pISSN: 0175-7598, eISSN: 1432-0614)
| ○ISSN (任意): | □ | 1432-0614
ISSN: 0175-7598
(pISSN: 0175-7598, eISSN: 1432-0614) Title: Applied microbiology and biotechnologyTitle(ISO): Appl Microbiol BiotechnolSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 80
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 597 604
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| ○年月日 (必須): | □ | 西暦 2008年 6月 27日 (平成 20年 6月 27日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00253-008-1563-6 (→Scopusで検索)
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| ○PMID (任意): | □ | 18584170 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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