| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | 櫻谷 英治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 2. | (英) Hirano Yuriko (日) (読)
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| 3. | (英) Kamada Nozomu (日) (読)
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| 4. | (英) Nojiri Masutoshi (日) (読)
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| 5. | (英) Ogawa Jun (日) (読)
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| 6. | (英) Shimizu Sakayu (日) (読)
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| ○題名 (必須): | □ | (英) Improvement of arachidonic acid production by mutants with lower n-3 desaturation activity derived from Mortierella alpina. (日)
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| ○要約 (任意): | □ | (英) Five mutants were obtained, Y11, Y135, Y164, Y180 and Y61, capable of accumulating higher amounts of arachidonic acid (AA) than Mortierella alpina 1S-4, an industrial strain for the production of AA-rich triacylglycerol (TG). This is thought to be due to low or no activity of n-3 desaturation with conversion of AA to eicosapentaenoic acid, which functions at a cultural temperature below 20 degrees C. In small-scale cultivation under optimum conditions, Y11 and Y61 respectively accumulated 4.97 mg/ml and 4.11 mg/ml of AA, using a high concentration of glucose at 20 degrees C, compared with 3.74 mg/ml for M. alpina 1S-4. In a 5-1 jar fermentor, the AA content in Y11 and Y61 kept increasing during cultivation, with consumption of the glucose in the medium; and this reached 1.48 mg/ml and 1.77 mg/ml (118 mg/g, 120 mg/g of dry mycelia) at day 10, respectively, compared with 0.95 mg/ml (86 mg/g of dry mycelia) for M. alpina 1S-4. From the results of lipid analysis, the TG contents of Y11 and Y61 in the major lipids were significantly higher than that of M. alpina 1S-4; and the AA percentages in TG of Y11 and Y61 were also higher. Both Y11 and Y61 are potential producers of TG rich in AA. (日)
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| ○キーワード (推奨): | 1. | (英) Arachidonic Acids (日) (読)
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| 2. | (英) Fatty Acid Desaturases (日) (読)
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| 3. | (英) Glucose (日) (読)
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| 4. | (英) Mortierella (日) (読)
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| 5. | (英) Mutation (日) (読)
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| 6. | (英) Temperature (日) (読)
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| 7. | (英) Triglycerides (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Applied Microbiology and Biotechnology ([Springer-Verlag])
(pISSN: 0175-7598, eISSN: 1432-0614)
| ○ISSN (任意): | □ | 0175-7598
ISSN: 0175-7598
(pISSN: 0175-7598, eISSN: 1432-0614) Title: Applied microbiology and biotechnologyTitle(ISO): Appl Microbiol BiotechnolSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 66
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 243 248
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| ○年月日 (必須): | □ | 西暦 2004年 12月 初日 (平成 16年 12月 初日)
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| ○DOI (任意): | □ | 10.1007/s00253-004-1682-7 (→Scopusで検索)
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| ○PMID (任意): | □ | 15300418 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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