| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○著者 (必須): | 1. | 櫻谷 英治 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース])
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| 2. | (英) Murata Shoichi (日) (読)
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| 3. | (英) Kanamaru Hiroyuki (日) (読)
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| 4. | (英) Shimizu Sakayu (日) (読)
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| ○題名 (必須): | □ | (英) Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4. (日)
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| ○要約 (任意): | □ | (英) We describe the isolation and characterization of a gene (MAELO) that encodes a fatty acid elongase from arachidonic acid-producing fungus Mortierella alpina 1S-4. Although the homologous MAELO gene had already been isolated from M. alpina ATCC 32221, its function had not yet been identified. The MAELO gene from M. alpina 1S-4 was confirmed to encode a fatty acid elongase by its expression in yeast Saccharomyces cerevisiae. Analysis of the fatty acid composition of the yeast transformant revealed the accumulation of 22-, 24-, and 26-carbon saturated fatty acids. On the other hand, RNA interference of the MAELO gene in M. alpina 1S-4 was carried out. The gene-silenced strain obtained on RNA interference exhibited low contents of 20-, 22-, and 24-carbon saturated fatty acids and a high content of stearic acid (18 carbons), compared with those in the wild strain. The enzyme encoded by the MAELO gene was demonstrated to be involved in the biosynthesis of 20-, 22-, and 24-carbon saturated fatty acids in M. alpina 1S-4. (日)
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| ○キーワード (推奨): | 1. | (英) Acetyltransferases (日) (読)
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| 2. | (英) Amino Acid Sequence (日) (読)
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| 3. | (英) Arachidonic Acid (日) (読)
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| 4. | (英) Fatty Acids (日) (読)
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| 5. | (英) Fungal Proteins (日) (読)
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| 6. | (英) Gene Expression (日) (読)
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| 7. | (英) Molecular Sequence Data (日) (読)
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| 8. | (英) Mortierella (日) (読)
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| 9. | (英) RNA Interference (日) (読)
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| 10. | (英) Saccharomyces cerevisiae (日) (読)
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| 11. | (英) Sequence Homology, Amino Acid (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Applied Microbiology and Biotechnology ([Springer-Verlag])
(pISSN: 0175-7598, eISSN: 1432-0614)
| ○ISSN (任意): | □ | 1432-0614
ISSN: 0175-7598
(pISSN: 0175-7598, eISSN: 1432-0614) Title: Applied microbiology and biotechnologyTitle(ISO): Appl Microbiol BiotechnolSupplier: Springer Online Journal ArchivePublisher: Springer (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 81
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 497 503
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| ○年月日 (必須): | □ | 西暦 2008年 9月 16日 (平成 20年 9月 16日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s00253-008-1675-z (→Scopusで検索)
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| ○PMID (任意): | □ | 18795285 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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