『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=284933)

EID=284933EID:284933, Map:0, LastModified:2015年11月12日(木) 15:14:48, Operator:[山中 絹代], Avail:TRUE, Censor:0, Owner:[白井 昭博], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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組織 (推奨): 1.徳島大学.工学部.生物工学科.生物機能工学講座 [継承]
著者 (必須): 1.白井 昭博 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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2.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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3.間世田 英明
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4.大政 健史
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題名 (必須): (英) Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-A light  (日)    [継承]
副題 (任意):
要約 (任意): (英) Ultraviolet-A (UV-A) can damage microbes by generating reactive oxygen species (ROS), singlet oxygen, superoxides, hydrogen peroxide and hydroxyl radicals. These species readily react with lipids, proteins, DNA and other constituents of cells, leading to oxidative deterioration and the eventual death of the microbe. However, the oxidative ability of these reactive species also harms the viability of mammalian cells such as fibroblasts and keratinocytes, as they cause both acute and chronic damage, photo-aging, and photo-carcinogenesis. This study describes a UV-A treatment that does not affect the viability or growth of human neonate dermal fibroblasts, as determined by examining the post-irradiation cell density after the addition of polyphenols as antioxidants. The results demonstrate the possible wide applicability of UV-A sterilization. The potency of polyphenols for attenuating UV-A-induced ROS generation in cells was tested using (+)-catechin hydrate, (-)- epigallocatechin gallate hydrate, morin hydrate, quercetin hydrate and resveratrol. The lowest concentration of polyphenols required to reduce ROS by 50% in cells upon exposure to a dose of 15 J cm-2 was determined and defined as its IC50. Pre-treatment with morin hydrate at its IC50 allowed cells irradiated with 5.0 J cm-2 UV-A to recover to the level of the specific growth rate of cells incubated without UV-A irradiation. However, the growth rate of cells exposed to 15 J cm-2 UV-A irradiation was scarcely influenced by co-incubation with morin hydrate; this dose of UV-A also suppressed cell growthcompletely in the absence of morin hydrate, although co-incubation resulted in no decrease in cell viability. This study demonstrates the potential of polyphenols for protecting both the viability of cells and their ability to proliferate from damage caused by UV-A-irradiation.  (日)    [継承]
キーワード (推奨): 1. (英) Polyphenol (日) (読) [継承]
2. (英) Ultraviolet-A (日) (読) [継承]
3. (英) Reactive oxygen species (日) (読) [継承]
4. (英) Cell proliferation (日) (読) [継承]
発行所 (推奨): 日本防菌防黴学会 [継承]
誌名 (必須): Biocontrol Science ([日本防菌防黴学会])
(pISSN: 1342-4815, eISSN: 1884-0205)

ISSN (任意): 1884-0205
ISSN: 1342-4815 (pISSN: 1342-4815, eISSN: 1884-0205)
Title: Biocontrol science
Title(ISO): Biocontrol Sci
Supplier: 日本防菌防黴学会
Publisher: Society for Antibacterial and Antifungal Agents
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 20 [継承]
(必須): 1 [継承]
(必須): 27 33 [継承]
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年月日 (必須): 西暦 2015年 3月 10日 (平成 27年 3月 10日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/130005061978/ [継承]
DOI (任意): 10.4265/bio.20.27    (→Scopusで検索) [継承]
PMID (任意): 25817810    (→Scopusで検索) [継承]
NAID (任意): 130005061978 [継承]
WOS (任意): 000352754800004 [継承]
Scopus (任意): 2-s2.0-84926366827 [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

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和文冊子 ● Akihiro Shirai, Masayoshi Onitsuka, Hideaki Maseda and Takeshi Omasa : Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-A light, Biocontrol Science, Vol.20, No.1, 27-33, 2015.
欧文冊子 ● Akihiro Shirai, Masayoshi Onitsuka, Hideaki Maseda and Takeshi Omasa : Effect of polyphenols on reactive oxygen species production and cell growth of human dermal fibroblasts after irradiation with ultraviolet-A light, Biocontrol Science, Vol.20, No.1, 27-33, 2015.

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