| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 阿部 佳織
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| 2. | (英) Hashimoto Y (日) (読)
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| 3. | (英) Yatsushiro S (日) (読)
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| 4. | (英) Yamamura S (日) (読)
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| 5. | 板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 6. | 廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| 7. | 木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
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| 8. | (英) Tanaka M (日) (読)
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| 9. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| 10. | 大家 利彦 (産業技術総合研究所健康工学研究センター)
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| 11. | 馬場 嘉信
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| 12. | 片岡 正俊
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| ○題名 (必須): | □ | (英) Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip. (日)
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| ○要約 (任意): | □ | (英) Sandwich enzyme-linked immunosorbant assay (ELISA) using a 96-well plate is frequently employed for clinical diagnosis, but is time-and sample-consuming. To overcome these drawbacks, we performed a sandwich ELISA on a microchip. The microchip was made of cyclic olefin copolymer with 4 straight microchannels. For the construction of the sandwich ELISA for interleukin-6 (IL-6) or tumor necrosis factor-α (TNF-α), we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st anti-IL-6 antibody or 1st anti-TNF-α antibody on the surface of the each microchannel. After the infusion of 2 µl of sample to the microchannel and a 20 min incubation, 2 µl of biotinylated 2nd antibody for either antigen was infused and a 10 min incubation. Then 2 µl of avidin-horseradish peroxidase was infused; and after a 5 min incubation, the substrate for peroxidase was infused, and the luminescence intensity was measured. Calibration curves were obtained between the concentration and luminescence intensity over the range of 0 to 32 pg/ml (IL-6: R(2) = 0.9994, TNF-α: R(2) = 0.9977), and the detection limit for each protein was 0.28 pg/ml and 0.46 pg/ml, respectively. Blood IL-6 and TNF-α concentrations of 5 subjects estimated from the microchip data were compared with results obtained by the conventional method, good correlations were observed between the methods according to linear regression analysis (IL-6: R(2) = 0.9954, TNF-α: R(2) = 0.9928). The reproducibility of the presented assay for the determination of the blood IL-6 and TNF-α concentration was comparable to that obtained with the 96-well plate. Simultaneous detection of blood IL-6 and TNF-α was possible by the deposition and fixation of each 1st antibody on the surface of a separate microchannel. This assay enabled us to determine simultaneously blood IL-6 and TNF-α with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and will be applicable to clinic diagnosis. (日)
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| ○キーワード (推奨): | 1. | (英) Enzyme-Linked Immunosorbent Assay (日) (読)
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| 2. | (英) Humans (日) (読)
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| 3. | (英) Immunoassay (日) (読)
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| 4. | (英) Interleukin-6 (日) (読)
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| 5. | (英) Lab-On-A-Chip Devices (日) (読)
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| 6. | (英) Luminescent Measurements (日) (読)
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| 7. | (英) Reproducibility of Results (日) (読)
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| 8. | (英) Tumor Necrosis Factor-alpha (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 8
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | e53620 e53620
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| ○年月日 (必須): | □ | 西暦 2013年 2月 初日 (平成 25年 2月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0053620 (→Scopusで検索)
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| ○PMID (任意): | □ | 23326472 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000313551500087
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| ○Scopus (任意): | □ | 2-s2.0-84872235277
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0053620 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) OtherID: PMC3541141 (日)
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