『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=280269)

EID=280269EID:280269, Map:0, LastModified:2014年10月28日(火) 11:30:10, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[木戸 淳一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.阿部 佳織
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Hashimoto Y (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Yatsushiro S (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Yamamura S (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5.板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6.廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7.木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Tanaka M (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
9.篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
10.大家 利彦 (産業技術総合研究所健康工学研究センター)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
11.馬場 嘉信
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
12.片岡 正俊
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Sandwich enzyme-linked immunosorbant assay (ELISA) using a 96-well plate is frequently employed for clinical diagnosis, but is time-and sample-consuming. To overcome these drawbacks, we performed a sandwich ELISA on a microchip. The microchip was made of cyclic olefin copolymer with 4 straight microchannels. For the construction of the sandwich ELISA for interleukin-6 (IL-6) or tumor necrosis factor-α (TNF-α), we used a piezoelectric inkjet printing system for the deposition and fixation of the 1st anti-IL-6 antibody or 1st anti-TNF-α antibody on the surface of the each microchannel. After the infusion of 2 µl of sample to the microchannel and a 20 min incubation, 2 µl of biotinylated 2nd antibody for either antigen was infused and a 10 min incubation. Then 2 µl of avidin-horseradish peroxidase was infused; and after a 5 min incubation, the substrate for peroxidase was infused, and the luminescence intensity was measured. Calibration curves were obtained between the concentration and luminescence intensity over the range of 0 to 32 pg/ml (IL-6: R(2) = 0.9994, TNF-α: R(2) = 0.9977), and the detection limit for each protein was 0.28 pg/ml and 0.46 pg/ml, respectively. Blood IL-6 and TNF-α concentrations of 5 subjects estimated from the microchip data were compared with results obtained by the conventional method, good correlations were observed between the methods according to linear regression analysis (IL-6: R(2) = 0.9954, TNF-α: R(2) = 0.9928). The reproducibility of the presented assay for the determination of the blood IL-6 and TNF-α concentration was comparable to that obtained with the 96-well plate. Simultaneous detection of blood IL-6 and TNF-α was possible by the deposition and fixation of each 1st antibody on the surface of a separate microchannel. This assay enabled us to determine simultaneously blood IL-6 and TNF-α with accuracy, satisfactory sensitivity, time saving ability, and low consumption of sample and reagents, and will be applicable to clinic diagnosis.  (日)    [継承]
キーワード (推奨): 1. (英) Enzyme-Linked Immunosorbent Assay (日) (読) [継承]
2. (英) Humans (日) (読) [継承]
3. (英) Immunoassay (日) (読) [継承]
4. (英) Interleukin-6 (日) (読) [継承]
5. (英) Lab-On-A-Chip Devices (日) (読) [継承]
6. (英) Luminescent Measurements (日) (読) [継承]
7. (英) Reproducibility of Results (日) (読) [継承]
8. (英) Tumor Necrosis Factor-alpha (日) (読) [継承]
発行所 (推奨):
誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 8 [継承]
(必須): 1 [継承]
(必須): e53620 e53620 [継承]
都市 (任意):
年月日 (必須): 西暦 2013年 2月 初日 (平成 25年 2月 初日) [継承]
URL (任意):
DOI (任意): 10.1371/journal.pone.0053620    (→Scopusで検索) [継承]
PMID (任意): 23326472    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意): 000313551500087 [継承]
Scopus (任意): 2-s2.0-84872235277 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.pone.0053620  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) OtherID: PMC3541141  (日)    [継承]

標準的な表示

和文冊子 ● Kaori Abe, Y Hashimoto, S Yatsushiro, S Yamamura, Mika Bandou, Yuka Hiroshima, Jun-ichi Kido, M Tanaka, Yasuo Shinohara, Toshihiko Ooie, Yoshinobu Baba and Masatoshi Kataoka : Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip., PLoS ONE, Vol.8, No.1, e53620, 2013.
欧文冊子 ● Kaori Abe, Y Hashimoto, S Yatsushiro, S Yamamura, Mika Bandou, Yuka Hiroshima, Jun-ichi Kido, M Tanaka, Yasuo Shinohara, Toshihiko Ooie, Yoshinobu Baba and Masatoshi Kataoka : Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip., PLoS ONE, Vol.8, No.1, e53620, 2013.

関連情報

Number of session users = 0, LA = 2.85, Max(EID) = 375544, Max(EOID) = 1006332.