『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=276959)

EID=276959EID:276959, Map:0, LastModified:2018年4月20日(金) 19:12:41, Operator:[白井 昭博], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Tatsuzawa Miki (日) (読)
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学籍番号 (推奨): **** [ユーザ]
[継承]
3. (英) Asano Ryutaro (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Kumagai Izumi (日) (読)
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学籍番号 (推奨):
[継承]
5.白井 昭博 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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学籍番号 (推奨):
[継承]
6.間世田 英明
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学籍番号 (推奨):
[継承]
7.大政 健史
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) The aggregation of therapeutic antibodies during the manufacturing process is problematic because of the potential risks posed by the aggregates, such as an unexpected immune response. One of the hallmark effects of trehalose, a disaccharide consisting of two alpha-glucose units, is as a chemical chaperone with anti-aggregation activity. In this study, Chinese hamster ovary (CHO) cell line producing a diabody-type bispecific antibody were cultured in medium containing trehalose and the aggregation of the secreted proteins during the culture process was analyzed. An analysis of the various forms of the antibody (monomeric, dimeric, and large aggregates) showed that trehalose decreased the relative content of large aggregates by two thirds. The aggregation kinetics indicated that trehalose directly inhibited the polymerization and aggregation steps in a nucleation-dependent aggregation mechanism. Moreover, both specific and volumetric antibody production were increased in CHO cells cultured in trehalose-containing medium. Thus, the addition of trehalose to recombinant CHO cell cultures would offer a practical strategy for quality improvement in the production of therapeutic antibodies.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antibodies, Bispecific (日) (読) [継承]
3. (英) Bioreactors (日) (読) [継承]
4. (英) CHO Cells (日) (読) [継承]
5. (英) Cricetinae (日) (読) [継承]
6. (英) Cricetulus (日) (読) [継承]
7. (英) Culture Media (日) (読) [継承]
8. (英) Kinetics (日) (読) [継承]
9. (英) Protein Binding (日) (読) [継承]
10. (英) Trehalose (日) (読) [継承]
発行所 (推奨): (英) The Society for Biotechnology, Japan (日) 公益社団法人日本生物工学会 (読) [継承]
誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 117 [継承]
(必須): 5 [継承]
(必須): 632 638 [継承]
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年月日 (必須): 西暦 2014年 5月 初日 (平成 26年 5月 初日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/110009823142/ [継承]
DOI (任意): 10.1016/j.jbiosc.2013.10.022    (→Scopusで検索) [継承]
PMID (任意): 24315529    (→Scopusで検索) [継承]
NAID (任意): 110009823142 [継承]
WOS (任意): 000337653600018 [継承]
Scopus (任意): 2-s2.0-84897055074 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1016/j.jbiosc.2013.10.022  (日)    [継承]
2.(英) Article.ELocationID: S1389-1723(13)00399-X  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) KeywordList.Keyword: Antibody aggregation  (日)    [継承]
6.(英) KeywordList.Keyword: Antibody production  (日)    [継承]
7.(英) KeywordList.Keyword: Bispecific diabody  (日)    [継承]
8.(英) KeywordList.Keyword: Cell culture  (日)    [継承]
9.(英) KeywordList.Keyword: Chemical chaperone  (日)    [継承]
10.(英) KeywordList.Keyword: Chinese hamster ovary cell  (日)    [継承]
11.(英) KeywordList.Keyword: Trehalose  (日)    [継承]

標準的な表示

和文冊子 ● Masayoshi Onitsuka, Miki Tatsuzawa, Ryutaro Asano, Izumi Kumagai, Akihiro Shirai, Hideaki Maseda and Takeshi Omasa : Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells, Journal of Bioscience and Bioengineering, Vol.117, No.5, 632-638, 2014.
欧文冊子 ● Masayoshi Onitsuka, Miki Tatsuzawa, Ryutaro Asano, Izumi Kumagai, Akihiro Shirai, Hideaki Maseda and Takeshi Omasa : Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells, Journal of Bioscience and Bioengineering, Vol.117, No.5, 632-638, 2014.

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