『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=276943)

EID=276943EID:276943, Map:0, LastModified:2016年12月22日(木) 14:59:47, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Kawaguchi Akira (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Asano Ryutaro (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Kumagai Izumi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Honda Kohsuke (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Ohtake Hisao (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
7.大政 健史
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture  (日)    [継承]
副題 (任意):
要約 (任意): (英) N-Glycosylation of therapeutic antibodies contributes not only to their biological function, but also to their stability and tendency to aggregate. Here, we investigated the impact of the glycosylation status of an aggregated antibody that accumulated during the bioreactor culture of Chinese hamster ovary cells. High-performance liquid chromatography analysis showed that there was no apparent difference in the glycosylation patterns of monomeric, dimeric, and large aggregated forms of the antibody. In contrast, lectin binding assays, which enable the total amounts of specific sugar residues to be detected, showed that both galactose and fucose residues in dimers and large aggregates were reduced to 70-80% of the amount in monomers. These results strongly suggest that the lack of N-linked oligosaccharides, a result of deglycosylation or aglycosylation, occurred in a proportion of the dimeric and large aggregated components. The present study demonstrates that glycosylation heterogeneities are a potential cause of antibody aggregation in cell culture of Chinese hamster ovary cells, and that the lack of N-glycosylation promotes the formation of dimers and finally results in large aggregates.  (日)    [継承]
キーワード (推奨): 1. (英) Chinese hamster ovary cell (日) (読) [継承]
2. (英) Cell culture (日) (読) [継承]
3. (英) Antibody production (日) (読) [継承]
4. (英) Antibody aggregation (日) (読) [継承]
5. (英) Glycosylation heterogeneity (日) (読) [継承]
6. (英) Bispecific diabody (日) (読) [継承]
発行所 (推奨): 日本生物工学会 [継承]
誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1389-1723
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
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(必須): 117 [継承]
(必須): 5 [継承]
(必須): 639 644 [継承]
都市 (任意):
年月日 (必須): 西暦 2014年 5月 初日 (平成 26年 5月 初日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/40020068741/ [継承]
DOI (任意): 10.1016/j.jbiosc.2013.11.001    (→Scopusで検索) [継承]
PMID (任意): 24326352    (→Scopusで検索) [継承]
NAID (任意): 40020068741 [継承]
WOS (任意): 000337653600019 [継承]
Scopus (任意): 2-s2.0-84897065464 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1016/j.jbiosc.2013.11.001  (日)    [継承]
2.(英) Article.ELocationID: S1389-1723(13)00404-0  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) KeywordList.Keyword: Antibody aggregation  (日)    [継承]
6.(英) KeywordList.Keyword: Antibody production  (日)    [継承]
7.(英) KeywordList.Keyword: Bispecific diabody  (日)    [継承]
8.(英) KeywordList.Keyword: Cell culture  (日)    [継承]
9.(英) KeywordList.Keyword: Chinese hamster ovary cell  (日)    [継承]
10.(英) KeywordList.Keyword: Glycosylation heterogeneity  (日)    [継承]

標準的な表示

和文冊子 ● Masayoshi Onitsuka, Akira Kawaguchi, Ryutaro Asano, Izumi Kumagai, Kohsuke Honda, Hisao Ohtake and Takeshi Omasa : Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture, Journal of Bioscience and Bioengineering, Vol.117, No.5, 639-644, 2014.
欧文冊子 ● Masayoshi Onitsuka, Akira Kawaguchi, Ryutaro Asano, Izumi Kumagai, Kohsuke Honda, Hisao Ohtake and Takeshi Omasa : Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture, Journal of Bioscience and Bioengineering, Vol.117, No.5, 639-644, 2014.

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