| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.工学部.生物工学科.生物機能工学講座
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| ○著者 (必須): | 1. | 白井 昭博 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | 粟飯原 睦美 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系])
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| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
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| 3. | 髙橋 章 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])
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| 4. | 間世田 英明
| ○役割 (任意): | □ | (英) (日) 計画，研究指導
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| 5. | 大政 健史
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| ○題名 (必須): | □ | (英) Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet A light (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) This study examined the utility of synergistic disinfection employing a gemini-quaternary ammonium salt (a gemini-QUAT, namely 3,3'-(2,7-dioxaoctane)bis(1-decylpyridinium bromide)), as an organic biocide in combination with irradiation by an ultraviolet-A (UV-A) light-emitting diode (LED) with a peak wavelength of 365nm. The combined system represents a novel disinfection method utilizing facilitated in situ oxidation depending on overproduction of reactive oxygen species (ROS) triggered by the initial action of the gemini-QUAT on the bacterial membrane. We demonstrate that this combination decreased the viability of pathogenic bacteria in a significant and rapid manner, and depended on doses of the gemini-QUAT and the fluence: the viability of Escherichia coli was reduced by greater than 5.0-logs by the combination procedure, but the decrease in viability was only 2.3-logs for exposure to UV at the same fluence dose in the absence of the gemini-QUAT. Adding catalase as a radical scavenger decreased bacterial inactivation by the combined disinfection procedure. Flow cytometric analysis indicated superoxide and hydrogen peroxide overproduction within cells treated with the combined disinfection procedure. The excessive superoxide, detected only in the combined system, appeared to be generated by the action of the gemini-QUAT at the bacterial membrane, leading to excessive and rapid generation of ROS in the system. Our data strongly suggested that this ROS promoted bacterial membrane peroxidation during initial treatment by the combination method, resulting in increased oxidative modification of DNA. These oxidative reactions may play an important role in the efficacy of this disinfection procedure. (日)
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| ○キーワード (推奨): | 1. | (英) Anti-Bacterial Agents (日) (読)
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| 2. | (英) Bacteria (日) (読)
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| 3. | (英) Bacterial Load (日) (読)
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| 4. | (英) DNA (日) (読)
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| 5. | (英) Lipid Peroxidation (日) (読)
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| 6. | (英) Pyridinium Compounds (日) (読)
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| 7. | (英) Quaternary Ammonium Compounds (日) (読)
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| 8. | (英) Reactive Oxygen Species (日) (読)
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| 9. | (英) Ultraviolet Rays (日) (読)
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| ○発行所 (推奨): | □ | Elsevier (->組織[Elsevier Science])
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| ○誌名 (必須): | □ | Journal of Photochemistry and Photobiology B: Biology ([Elsevier])
(pISSN: 1011-1344, eISSN: 1873-2682)
| ○ISSN (任意): | □ | 1873-2682
ISSN: 1011-1344
(pISSN: 1011-1344, eISSN: 1873-2682) Title: Journal of photochemistry and photobiology. B, BiologyTitle(ISO): J Photochem Photobiol BPublisher: Elsevier BV (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 130
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| ○号 (必須): |
| ○頁 (必須): | □ | 226 233
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2014年 1月 5日 (平成 26年 1月 5日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.jphotobiol.2013.11.027 (→Scopusで検索)
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| ○PMID (任意): | □ | 24362318 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000332999700027
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| ○Scopus (任意): | □ | 2-s2.0-84890840874
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1016/j.jphotobiol.2013.11.027 (日)
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| 2. | (英) Article.ELocationID: S1011-1344(13)00279-0 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) KeywordList.Keyword: Bactericidal action (日)
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| 5. | (英) KeywordList.Keyword: Disinfection (日)
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| 6. | (英) KeywordList.Keyword: Gemini-quaternary ammonium salt (日)
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| 7. | (英) KeywordList.Keyword: Reactive oxygen species (日)
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| 8. | (英) KeywordList.Keyword: Ultraviolet-A (日)
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