『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=271318EID:271318, Map:0, LastModified:2013年11月28日(木) 13:33:36, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[牟礼 英生], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨): 博士課程学生による研究報告 [継承]
組織 (推奨):
著者 (必須): 1.牟礼 英生
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2.松﨑 和仁
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3. (英) Kitazato KT (日) 北里 慶子 (読)
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4.溝渕 佳史 ([徳島大学.病院.診療科.脳·神経·精神科.脳神経外科]/[徳島大学.大学院ヘルスバイオサイエンス研究部.神経情報医学部門.情報統合医学講座.脳神経外科学])
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5.桑山 一行
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6.影治 照喜
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7.永廣 信治
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題名 (必須): (英) Akt2 and Akt3 play a pivotal role in malignant gliomas  (日)    [継承]
副題 (任意):
要約 (任意): (英) Akt, one of the major downstream effectors of phosphatidylinositol 3-kinase, is hyper-expressed and activated in a variety of cancers including glioblastoma. However, the expression profiles of the Akt isoforms Akt1/PKBalpha, Akt2/PKBbeta, and Akt3/PKBgamma and their functional roles in malignant glioma are not well understood. Therefore, we examined the protein and mRNA expression patterns of Akt isoforms in tissues from human astrocytomas, glioblastomas, and non-neoplastic regions. We also explored the biological role of each Akt isoform in malignant glioma cells using RNA interference-mediated knock-down and the over-expression of plasmid DNA of each isoform. The expression of Akt1 protein and mRNA was similar in glioma and normal control tissues. Although the protein and mRNA level of Akt2 increased with the pathological grade of malignancy, the expression of Akt3 mRNA and protein decreased as the malignancy grade increased. In U87MG, T98G, and TGB cells, the down-regulation of Akt2 or Akt3 by RNA interference reduced the expression of the phosphorylated form of Bad, resulting in the induction of caspase-dependent apoptosis. Akt1 knock-down did not affect cell growth or survival. We first demonstrate that the over-expression of Akt2 or Akt3 down-regulated the expression of the other protein and that endogenous Akt3 protein showed high kinase activity in U87MG cells. Our data suggest that Akt2 and Akt3 play an important role in the viability of human malignant glioma cells. Targeting Akt2 and Akt3 may hold promise for the treatment of patients with gliomas.  (日)    [継承]
キーワード (推奨): 1.アポトーシス (apoptosis) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Brain Neoplasms (日) (読) [継承]
4.細胞分離 (cell separation) [継承]
5. (英) Flow Cytometry (日) (読) [継承]
6.遺伝子発現 (gene expression) [継承]
7. (英) Gene Expression Profiling (日) (読) [継承]
8. (英) Glioma (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10.免疫組織化学 (immunohistochemistry) [継承]
11. (英) Immunoprecipitation (日) (読) [継承]
12. (英) In Situ Nick-End Labeling (日) (読) [継承]
13. (英) Isoenzymes (日) (読) [継承]
14. (英) Proto-Oncogene Proteins c-akt (日) (読) [継承]
15. (英) RNA Interference (日) (読) [継承]
16. (英) RNA, Messenger (日) (読) [継承]
17. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Neuro-Oncology (Society for Neuro-Oncology)
(pISSN: 1522-8517, eISSN: 1523-5866)

ISSN (任意): 1523-5866
ISSN: 1522-8517 (pISSN: 1522-8517, eISSN: 1523-5866)
Title: Neuro-oncology
Title(ISO): Neuro Oncol
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 12 [継承]
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(必須): 221 232 [継承]
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年月日 (必須): 西暦 2010年 3月 初日 (平成 22年 3月 初日) [継承]
URL (任意):
DOI (任意): 10.1093/neuonc/nop026    (→Scopusで検索) [継承]
PMID (任意): 20167810    (→Scopusで検索) [継承]
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WOS (任意): 000275708200002 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1093/neuonc/nop026  (日)    [継承]
2.(英) Article.Affiliation: Department of Neurosurgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto-cho, Tokushima, Tokushima 770-8503, Japan. gaudi0529@ybb.ne.jp  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC2940586  (日)    [継承]

標準的な表示

和文冊子 ● Hideo Mure, Kazuhito Matsuzaki, KT Kitazato, Yoshifumi Mizobuchi, Kazuyuki Kuwayama, Teruyoshi Kageji and Shinji Nagahiro : Akt2 and Akt3 play a pivotal role in malignant gliomas, Neuro-Oncology, Vol.12, No.3, 221-232, 2010.
欧文冊子 ● Hideo Mure, Kazuhito Matsuzaki, KT Kitazato, Yoshifumi Mizobuchi, Kazuyuki Kuwayama, Teruyoshi Kageji and Shinji Nagahiro : Akt2 and Akt3 play a pivotal role in malignant gliomas, Neuro-Oncology, Vol.12, No.3, 221-232, 2010.

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