『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=271009)

EID=271009EID:271009, Map:0, LastModified:2015年2月9日(月) 14:35:48, Operator:[松井 栄里], Avail:TRUE, Censor:0, Owner:[安部 秀斉], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Torikoshi Kazuo (日) (読)
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[継承]
2.安部 秀斉
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[継承]
3. (英) Matsubara Takeshi (日) (読)
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[継承]
4. (英) Hirano Takahiro (日) (読)
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[継承]
5. (英) Ohshima Takayuki (日) (読)
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[継承]
6.村上 太一
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学籍番号 (推奨):
[継承]
7. (英) Araki Makoto (日) (読)
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学籍番号 (推奨):
[継承]
8.美馬 晶
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学籍番号 (推奨):
[継承]
9. (英) Iehara Noriyuki (日) (読)
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[継承]
10. (英) Fukatsu Atsushi (日) (読)
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[継承]
11. (英) Kita Toru (日) (読)
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[継承]
12. (英) Arai Hidenori (日) (読)
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[継承]
13.土井 俊夫
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Protein Inhibitor of Activated STAT, PIASy Regulates α-Smooth Muscle Actin Expression by Interacting with E12 in Mesangial Cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Phenotypic transformation of mesangial cells (MCs) is implicated in the development of glomerular disease; however, the mechanisms underlying their altered genetic program is still unclear. α-smooth muscle actin (α-SMA) is known to be a crucial marker for phenotypic transformation of MCs. Recently, E-boxes and the class I basic helix-loop-helix proteins, such as E12 have been shown to regulateα-SMA expression. Therefore, we tried to identify a novel E12 binding protein in MCs and to examine its role in glomerulonephritis. We found that PIASy, one of the protein inhibitors of activated STAT family protein, interacted with E12 by yeast two-hybrid screens and coimmunopreciptation assays. Overexpression of E12 significantly enhanced theα-SMA promoter activity, and the increase was blocked by co-transfection of PIASy, but not by a PIASy RING mutant. In vivo sumoylation assays revealed that PIASy was a SUMO E3 ligase for E12. Furthermore, transforming growth factor-β (TGF-β) treatment induced expression of both PIASy and E12, consistent with α-SMA expression. Moreover, reduced expression of PIASy protein by siRNA specific for PIASy resulted in increased TGF-β-mediated α-SMA expression. In vivo, PIASy and E12 were dramatically upregulated along with α-SMA and TGF-β in the proliferative phase of Thy1 glomerulonephritis. Furthermore, an association between PIASy and E12 proteins was observed at day 6 by IP-western blotting, but not at day 0. These results suggest that TGF-β up-regulates PIASy expression in MCs to down-regulateα-SMA gene transcription by the interaction with E12.  (日)    [継承]
キーワード (推奨): 1. (英) Actins (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) COS Cells (日) (読) [継承]
5. (英) Cell Line (日) (読) [継承]
6. (英) Cell Proliferation (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8.免疫組織化学 (immunohistochemistry) [継承]
9. (英) Immunoprecipitation (日) (読) [継承]
10. (英) Male (日) (読) [継承]
11. (英) Mesangial Cells (日) (読) [継承]
12. (英) Protein Binding (日) (読) [継承]
13. (英) Protein Inhibitors of Activated STAT (日) (読) [継承]
14. (英) RNA Interference (日) (読) [継承]
15. (英) Rats (日) (読) [継承]
16. (英) Rats, Inbred WKY (日) (読) [継承]
17. (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読) [継承]
18. (英) Transcription Factor 3 (日) (読) [継承]
19. (英) Two-Hybrid System Techniques (日) (読) [継承]
発行所 (推奨):
誌名 (必須): PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)

ISSN (任意): 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 7 [継承]
(必須): 7 [継承]
(必須): e41186 e41186 [継承]
都市 (任意):
年月日 (必須): 西暦 2012年 7月 19日 (平成 24年 7月 19日) [継承]
URL (任意): http://ci.nii.ac.jp/naid/120004770223/ [継承]
DOI (任意): 10.1371/journal.pone.0041186    (→Scopusで検索) [継承]
PMID (任意): 22829926    (→Scopusで検索) [継承]
NAID (任意): 120004770223 [継承]
WOS (任意): 000306956300065 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1371/journal.pone.0041186  (日)    [継承]
2.(英) Article.Affiliation: Department of Nephrology, Kyoto University Graduate School of Medicine, Kyoto, Japan.  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC3400623  (日)    [継承]

標準的な表示

和文冊子 ● Kazuo Torikoshi, Hideharu Abe, Takeshi Matsubara, Takahiro Hirano, Takayuki Ohshima, Taichi Murakami, Makoto Araki, Akira Mima, Noriyuki Iehara, Atsushi Fukatsu, Toru Kita, Hidenori Arai and Toshio Doi : Protein Inhibitor of Activated STAT, PIASy Regulates α-Smooth Muscle Actin Expression by Interacting with E12 in Mesangial Cells., PLoS ONE, Vol.7, No.7, e41186, 2012.
欧文冊子 ● Kazuo Torikoshi, Hideharu Abe, Takeshi Matsubara, Takahiro Hirano, Takayuki Ohshima, Taichi Murakami, Makoto Araki, Akira Mima, Noriyuki Iehara, Atsushi Fukatsu, Toru Kita, Hidenori Arai and Toshio Doi : Protein Inhibitor of Activated STAT, PIASy Regulates α-Smooth Muscle Actin Expression by Interacting with E12 in Mesangial Cells., PLoS ONE, Vol.7, No.7, e41186, 2012.

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