『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須):
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Lei XiaoFeng (日) (読)
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学籍番号 (推奨):
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2. (英) Fujiwara Yukio (日) (読)
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[継承]
3. (英) Chang C. Y. Catherine (日) (読)
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4. (英) Chang Ta-Yuan (日) (読)
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[継承]
5. (英) Takeya Motohiro (日) (読)
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6.坂下 直実
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題名 (必須): (英) Association of ACAT1-positive vesicles with late endosomes/ lysosomes in cholesterol-rich human macrophages.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Acyl-coenzyme A: cholesterol acyltransferase1 (ACAT1) is an endoplasmic reticulum (ER)-resident enzyme that catalyzes the conversion of cholesterol into cholesteryl esters. We previously showed that cholesterol-loaded macrophages produce numerous ER-derived vesicles with elevated ACAT1 enzyme activity; some of these vesicles were shown to be closely associated with Golgi-related organelle(s). The aim of this study was to investigate the translocation of ACAT1 vesicle in cholesterol-loaded macrophages. To demonstrate association of ACAT1 with late endosomes/lysosomes (LE/LS), primary human macrophages with or without cholesterol-loading was subjected to confocal microscopy, immunoelectron microscopy, subcellular fractionation, and immunoadsorption assay. Furthermore, cholesterol esterification assay was also carried out to investigate function of ACAT1 associated LE/LS. Confocal fluorescence microscopy revealed that no significant ACAT1 signal was associated with the signal for LAMP2, a marker protein for LE/LS, in cholesterol non-loaded macrophages; however, approximately 20% of the total ACAT1 signals colocalized with the LAMP2 signal in cholesterol-loaded macrophages. ACAT1-positive membranes isolated by immunoadsorption using ACAT1-specific antibody contained LAMP2, demonstrating the association of ACAT1 and LE/LS. In addition, in macrophages phagocytosing latex beads, the close association of ACAT1 with LE/LS can be demonstrated in phagosomes isolated from cholesterol-loaded macrophages, not from non-loaded macrophages. Furthermore, cholesterol-loaded macrophages re-esterified aggregated LDL-derived cholesteryl ester even in the presence of U18666A, a reagent known to block egression of cholesterol from LE/LS. Our results indicated that cholesterol-loaded human macrophages produce LE/LS in close association with ACAT1, and may promote efficient esterification of modified LDL-derived free cholesterol on LE/LS.  (日)    [継承]
キーワード (推奨): 1. (英) Acetyl-CoA C-Acetyltransferase (日) (読) [継承]
2. (英) Animals (日) (読) [継承]
3. (英) Cells, Cultured (日) (読) [継承]
4. (英) Cholesterol (日) (読) [継承]
5. (英) Endoplasmic Reticulum (日) (読) [継承]
6. (英) Endosomes (日) (読) [継承]
7. (英) Esterification (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Immunoblotting (日) (読) [継承]
10. (英) Lysosomal-Associated Membrane Protein 2 (日) (読) [継承]
11. (英) Lysosomes (日) (読) [継承]
12. (英) Macrophages (日) (読) [継承]
13. (英) Mice (日) (読) [継承]
14. (英) Subcellular Fractions (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Atherosclerosis and Thrombosis (日本動脈硬化学会)
(pISSN: 1340-3478, eISSN: 1880-3873)

ISSN (任意): 1880-3873
ISSN: 1340-3478 (pISSN: 1340-3478, eISSN: 1880-3873)
Title: Journal of atherosclerosis and thrombosis
Title(ISO): J Atheroscler Thromb
Supplier: 一般社団法人 日本動脈硬化学会
Publisher: Japan Atherosclerosis Society
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 17 [継承]
(必須): 7 [継承]
(必須): 740 750 [継承]
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年月日 (必須): 西暦 2010年 6月 2日 (平成 22年 6月 2日) [継承]
URL (任意):
DOI (任意): 10.5551/jat.4416    (→Scopusで検索) [継承]
PMID (任意): 20523008    (→Scopusで検索) [継承]
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WOS (任意): 000282824200009 [継承]
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備考 (任意): 1.(英) Article.Affiliation: Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● (種別) : XiaoFeng Lei, Yukio Fujiwara, Catherine C. Y. Chang, Ta-Yuan Chang, Motohiro Takeya and Naomi Sakashita : Association of ACAT1-positive vesicles with late endosomes/ lysosomes in cholesterol-rich human macrophages., Journal of Atherosclerosis and Thrombosis, Vol.17, No.7, 740-750, (発行所), (都市), June 2010.
欧文冊子 ● (種別) : XiaoFeng Lei, Yukio Fujiwara, Catherine C. Y. Chang, Ta-Yuan Chang, Motohiro Takeya and Naomi Sakashita : Association of ACAT1-positive vesicles with late endosomes/ lysosomes in cholesterol-rich human macrophages., Journal of Atherosclerosis and Thrombosis, Vol.17, No.7, 740-750, (発行所), (都市), June 2010.

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