| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 国際共著 (徳島大学内研究者と国外研究機関所属研究者との共同研究)
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| ○学究種別 (推奨): |
| ○組織 (推奨): | 1. | 徳島大学.大学院ソシオテクノサイエンス研究部.ライフシステム部門.生命情報工学 (2006年4月1日〜2016年3月31日)
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| ○著者 (必須): | 1. | (英) Inoue Masahiro (日) (読)
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| 2. | (英) Yasuda Kouichi (日) (読)
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| 3. | (英) Uemura Haruki (日) (読)
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| 4. | (英) Yasaka Natsumi (日) (読)
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| 5. | (英) Schnaufer Achim (日) (読)
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| 6. | 矢野 仁康
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| ○貢献度 (任意): |
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| 7. | 木戸 博 ([徳島大学.先端酵素学研究所.重点研究部門])
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| 8. | (英) Kohda Daisuke (日) (読)
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| 9. | (英) Doi Hirofumi (日) (読)
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| ○貢献度 (任意): |
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| 10. | (英) Fukuma Toshihide (日) (読)
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| 11. | 辻 明彦
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| 12. | (英) Horikoshi Nobuo (日) (読)
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| ○貢献度 (任意): |
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| ○題名 (必須): | □ | (英) Trypanosoma brucei 14-3-3I and II proteins predominantly form a heterodimer structure that acts as a potent cell cycle regulator in vivo. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Hetero- and homodimerization of 14-3-3 proteins demonstrate distinctive functions in mammals and plants. Trypanosoma brucei 14-3-3I and II (Tb14-3-3I and II) play pivotal roles in motility, cytokinesis and the cell cycle; however, the significance and the mechanism of Tb14-3-3 dimerization are remained to be elucidated. We found that ectopically expressed epitope-tagged Tb14-3-3I and II proteins formed hetero- and homodimers with endogenous Tb14-3-3I and II proteins. However, we also found the ability to form hetero- or homodimers between Tb14-3-3I and II proteins was clearly affected by the sequence and location of the epitope tag used. We found a blue native polyacrylamide gel electrophoresis system followed by western blotting may distinguish monomer from dimer structure, and stable from unstable conformation of Tb14-3-3. Combined with co-immunoprecipitation results, we revealed that Tb14-3-3 proteins mainly existed as heterodimeric form. Furthermore, co-overexpression of Tb14-3-3I and II proteins in T. brucei induced aberrant numbers of organelles in cells, but overexpression of either isoform alone rarely produced such morphology. These results suggest that heterodimers play more significant roles than homodimers not only in the maintenance of steady-state levels of the 14-3-3 proteins but also in the regulation of cytokinesis. (日)
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| ○キーワード (推奨): | 1. | (英) 14-3-3 Proteins (日) (読)
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| 2. | 細胞周期 (cell cycle)
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| 3. | 細胞質分裂 (cytokinesis)
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| 4. | (英) Humans (日) (読)
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| 5. | (英) Polymerase Chain Reaction (日) (読)
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| 6. | (英) Protozoan Proteins (日) (読)
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| 7. | (英) Trypanosoma brucei brucei (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)
| ○ISSN (任意): | □ | 1756-2651
ISSN: 0021-924X
(pISSN: 0021-924X, eISSN: 1756-2651) Title: Journal of biochemistryTitle(ISO): J BiochemSupplier: Oxford University PressPublisher: Oxford University Press (NLM Catalog)
(医中誌Web)
(J-STAGE)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 153
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| ○号 (必須): | □ | 5
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| ○頁 (必須): | □ | 431 439
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2013年 5月 初日 (平成 25年 5月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1093/jb/mvt016 (→Scopusで検索)
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| ○PMID (任意): | □ | 23457405 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000318561900005
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| ○Scopus (任意): | □ | 2-s2.0-84877278778
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| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1093/jb/mvt016 (日)
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| 2. | (英) Article.Affiliation: Department of Infectious Medicine, Division of Eukaryotic Microbiology, Kurume University School of medicine, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan. inouedna@med.kurume.u-ac.jp (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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