『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=265298)

EID=265298EID:265298, Map:0, LastModified:2014年8月20日(水) 12:57:47, Operator:[谷口 寿章], Avail:TRUE, Censor:0, Owner:[谷口 寿章], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hyodo Kiwamu (日) (読)
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2. (英) Mine Akira (日) (読)
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3.谷口 貴子
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4. (英) Kaido Masanori (日) (読)
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5. (英) Mise Kazuyuki (日) (読)
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6.谷口 寿章
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7. (英) Okuno Tetsuro (日) (読)
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題名 (必須): (英) ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Eukaryotic positive-strand RNA viruses replicate using the membrane-bound replicase complexes, which contain multiple viral and host components. Virus infection induces the remodeling of intracellular membranes. Virus-induced membrane structures are thought to increase the local concentration of the components that are required for replication and provide a scaffold for tethering the replicase complexes. However, the mechanisms underlying virus-induced membrane remodeling are poorly understood. RNA replication of red clover necrotic mosaic virus (RCNMV), a positive-strand RNA plant virus, is associated with the endoplasmic reticulum (ER) membranes, and ER morphology is perturbed in RCNMV-infected cells. Here, we identified ADP ribosylation factor 1 (Arf1) in the affinity-purified RCNMV RNA-dependent RNA polymerase fraction. Arf1 is a highly conserved, ubiquitous, small GTPase that is implicated in the formation of the coat protein complex I (COPI) vesicles on Golgi membranes. Using in vitro pulldown and bimolecular fluorescence complementation analyses, we showed that Arf1 interacted with the viral p27 replication protein within the virus-induced large punctate structures of the ER membrane. We found that inhibition of the nucleotide exchange activity of Arf1 using the inhibitor brefeldin A (BFA) disrupted the assembly of the viral replicase complex and p27-mediated ER remodeling. We also showed that BFA treatment and the expression of dominant negative Arf1 mutants compromised RCNMV RNA replication in protoplasts. Interestingly, the expression of a dominant negative mutant of Sar1, a key regulator of the biogenesis of COPII vesicles at ER exit sites, also compromised RCNMV RNA replication. These results suggest that the replication of RCNMV depends on the host membrane traffic machinery.  (日)    [継承]
キーワード (推奨): 1. (英) ADP-Ribosylation Factor 1 (日) (読) [継承]
2. (英) Arabidopsis (日) (読) [継承]
3. (英) Centrifugation (日) (読) [継承]
4.小胞体 (endoplasmic reticulum) [継承]
5. (英) Fluorescence (日) (読) [継承]
6. (英) Host-Pathogen Interactions (日) (読) [継承]
7. (英) Protein Binding (日) (読) [継承]
8. (英) Protein Interaction Mapping (日) (読) [継承]
9. (英) Tobacco (日) (読) [継承]
10. (英) Tombusviridae (日) (読) [継承]
11. (英) Viral Proteins (日) (読) [継承]
12. (英) Virus Replication (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Virology ([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)

ISSN (任意): 1098-5514
ISSN: 0022-538X (pISSN: 0022-538X, eISSN: 1098-5514)
Title: Journal of virology
Title(ISO): J Virol
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 87 [継承]
(必須): 1 [継承]
(必須): 163 176 [継承]
都市 (任意):
年月日 (必須): 西暦 2013年 1月 初日 (平成 25年 1月 初日) [継承]
URL (任意):
DOI (任意): 10.1128/JVI.02383-12    (→Scopusで検索) [継承]
PMID (任意): 23097452    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1128/JVI.02383-12  (日)    [継承]
2.(英) Article.Affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC3536388  (日)    [継承]

標準的な表示

和文冊子 ● Kiwamu Hyodo, Akira Mine, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Hisaaki Taniguchi and Tetsuro Okuno : ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus., Journal of Virology, Vol.87, No.1, 163-176, 2013.
欧文冊子 ● Kiwamu Hyodo, Akira Mine, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Hisaaki Taniguchi and Tetsuro Okuno : ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus., Journal of Virology, Vol.87, No.1, 163-176, 2013.

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