『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=265297)

EID=265297EID:265297, Map:0, LastModified:2014年8月20日(水) 12:58:42, Operator:[谷口 寿章], Avail:TRUE, Censor:0, Owner:[谷口 寿章], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Mine Akira (日) (読)
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学籍番号 (推奨):
[継承]
2. (英) Hyodo Kiwamu (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Tajima Yuri (日) (読)
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[継承]
4. (英) Kusumanegara Kusumawaty (日) (読)
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[継承]
5.谷口 貴子
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6. (英) Kaido Masanori (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Mise Kazuyuki (日) (読)
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8.谷口 寿章
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9. (英) Okuno Tetsuro (日) (読)
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題名 (必須): (英) Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Assembly of viral replicase complexes of eukaryotic positive-strand RNA viruses is a regulated process: multiple viral and host components must be assembled on intracellular membranes and ordered into quaternary complexes capable of synthesizing viral RNAs. However, the molecular mechanisms underlying this process are poorly understood. In this study, we used a model virus, Red clover necrotic mosaic virus (RCNMV), whose replicase complex can be detected readily as the 480-kDa functional protein complex. We found that host heat shock proteins Hsp70 and Hsp90 are required for RCNMV RNA replication and that they interact with p27, a virus-encoded component of the 480-kDa replicase complex, on the endoplasmic reticulum membrane. Using a cell-free viral translation/replication system in combination with specific inhibitors of Hsp70 and Hsp90, we found that inhibition of p27-Hsp70 interaction inhibits the formation of the 480-kDa complex but instead induces the accumulation of large complexes that are nonfunctional in viral RNA synthesis. In contrast, inhibition of p27-Hsp90 interaction did not induce such large complexes but rendered p27 incapable of binding to a specific viral RNA element, which is a critical step for the assembly of the 480-kDa replicase complex and viral RNA replication. Together, our results suggest that Hsp70 and Hsp90 regulate different steps in the assembly of the RCNMV replicase complex.  (日)    [継承]
キーワード (推奨): 1. (英) Cloning, Molecular (日) (読) [継承]
2.小胞体 (endoplasmic reticulum) [継承]
3. (英) Gene Silencing (日) (読) [継承]
4. (英) HSP70 Heat-Shock Proteins (日) (読) [継承]
5. (英) HSP90 Heat-Shock Proteins (日) (読) [継承]
6. (英) Microscopy, Confocal (日) (読) [継承]
7. (英) Plants (日) (読) [継承]
8. (英) Protein Binding (日) (読) [継承]
9. (英) Protein Biosynthesis (日) (読) [継承]
10. (英) RNA Replicase (日) (読) [継承]
11. (英) RNA Viruses (日) (読) [継承]
12. (英) RNA, Viral (日) (読) [継承]
13. (英) Tobacco (日) (読) [継承]
14. (英) Tombusviridae (日) (読) [継承]
15. (英) Virus Replication (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Virology ([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)

ISSN (任意): 1098-5514
ISSN: 0022-538X (pISSN: 0022-538X, eISSN: 1098-5514)
Title: Journal of virology
Title(ISO): J. Virol.
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 86 [継承]
(必須): 22 [継承]
(必須): 12091 12104 [継承]
都市 (任意):
年月日 (必須): 西暦 2012年 8月 29日 (平成 24年 8月 29日) [継承]
URL (任意):
DOI (任意): 10.1128/JVI.01659-12    (→Scopusで検索) [継承]
PMID (任意): 22933272    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1128/JVI.01659-12  (日)    [継承]
2.(英) Article.Affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
5.(英) OtherID: PMC3486462  (日)    [継承]

標準的な表示

和文冊子 ● Akira Mine, Kiwamu Hyodo, Yuri Tajima, Kusumawaty Kusumanegara, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Hisaaki Taniguchi and Tetsuro Okuno : Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus., Journal of Virology, Vol.86, No.22, 12091-12104, 2012.
欧文冊子 ● Akira Mine, Kiwamu Hyodo, Yuri Tajima, Kusumawaty Kusumanegara, Takako Taniguchi, Masanori Kaido, Kazuyuki Mise, Hisaaki Taniguchi and Tetsuro Okuno : Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus., Journal of Virology, Vol.86, No.22, 12091-12104, 2012.

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