『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=264440EID:264440, Map:0, LastModified:2013年11月29日(金) 14:29:15, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[木戸 淳一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
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著者 (必須): 1.木戸 淳一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
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[継承]
2.板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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[継承]
3.廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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[継承]
4. (英) Iwasaka Hiroyuki (日) 岩坂 博之 (読) いわさか ひろゆき
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5. (英) Yamada Keisuke (日) 山田 圭佑 (読) やまだ けいすけ
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6. (英) Ohgami Naoto (日) 大上 直人 (読) おおがみ なおと
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7. (英) Namubu Toshiyuki (日) 南部 敏之 (読) なんぶ としゆき
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8.片岡 正俊
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9.山本 武範
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10.篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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11.佐川 幾子 ([徳島大学.大学院医歯薬学研究部.医学域.総合研究支援センター])
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12.永田 俊彦
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題名 (必須): (英) Analysis of proteins in human gingival crevicular fluid by mass spectrometry  (日)    [継承]
副題 (任意):
要約 (任意): (英) Gingival crevicular fluid is a bodily fluid transuded from periodontal tissues into the gingival crevice and periodontal pocket, and contains many species of components. Proteins in gingival crevicular fluid have been studied as markers for periodontal diseases. Mass spectrometric analysis is used for the analyses of proteins, lipids, saccharides and metals, and expected as an approach for disease diagnosis. For better analysis of the protein components in gingival crevicular fluid, we investigated proteins in gingival crevicular fluid samples from the healthy gingival crevice and periodontal pocket using mass spectrometry. Gingival crevicular fluid samples were collected from subjects who gave their informed consent and were periodontally healthy or had diseased pockets. These samples were electrophoretically separated, and each fraction on the gels was analysed by nano liquid chromatography coupled with tandem mass spectrometry. Antimicrobial peptides detected in gingival crevicular fluid were confirmed by western blotting. One hundred and four proteins were detected in gingival crevicular fluid samples from both healthy sites and sites of periodontitis; 64 proteins were contained only in gingival crevicular fluid from healthy sites and 63 proteins were observed only in gingival crevicular fluid from periodontitis sites. These proteins were blood-, cytoskeleton-, immunity-, inflammation- and lipid-related proteins and enzymes. Some proteins, including ceruloplasmin, glycogen phosphorylase, glutathione S-transferase, phosphoglycerate mutase, psoriasin, S100A11 and resistin, were identified for the first time in gingival crevicular fluid. Antimicrobial peptides, such as lactoferrin, α1-antitrypsin, lipocalin, S100A7, S100A8, S100A9 and cathelicidin, were observed by mass spectrometry and western blotting. Multiple protein components in gingival crevicular fluid were analysed at the same time using mass spectrometry, and this approach may be useful for the diagnosis of periodontal diseases.  (日)    [継承]
キーワード (推奨): 1. (英) Adult (日) (読) [継承]
2. (英) Aged (日) (読) [継承]
3. (英) Antimicrobial Cationic Peptides (日) (読) [継承]
4. (英) Blotting, Western (日) (読) [継承]
5. (英) Case-Control Studies (日) (読) [継承]
6. (英) Ceruloplasmin (日) (読) [継承]
7. (英) Chromatography, Liquid (日) (読) [継承]
8. (英) Electrophoresis, Polyacrylamide Gel (日) (読) [継承]
9. (英) Female (日) (読) [継承]
10. (英) Gingival Crevicular Fluid (日) (読) [継承]
11. (英) Glutathione Transferase (日) (読) [継承]
12. (英) Glycogen Phosphorylase (日) (読) [継承]
13. (英) Humans (日) (読) [継承]
14. (英) Male (日) (読) [継承]
15. (英) Middle Aged (日) (読) [継承]
16. (英) Periodontal Pocket (日) (読) [継承]
17.歯周病 (periodontitis) [継承]
18. (英) Phosphoglycerate Mutase (日) (読) [継承]
19. (英) Proteins (日) (読) [継承]
20. (英) Resistin (日) (読) [継承]
21. (英) S100 Proteins (日) (読) [継承]
22. (英) Tandem Mass Spectrometry (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Journal of Periodontal Research ([Blackwell Publishing Ltd])
(pISSN: 0022-3484, eISSN: 1600-0765)

ISSN (任意): 1600-0765
ISSN: 0022-3484 (pISSN: 0022-3484, eISSN: 1600-0765)
Title: Journal of periodontal research
Title(ISO): J Periodontal Res
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 47 [継承]
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(必須): 488 499 [継承]
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年月日 (必須): 西暦 2012年 1月 3日 (平成 24年 1月 3日) [継承]
URL (任意):
DOI (任意): 10.1111/j.1600-0765.2011.01458.x    (→Scopusで検索) [継承]
PMID (任意): 22220998    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1111/j.1600-0765.2011.01458.x  (日)    [継承]
2.(英) Article.Affiliation: Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. kido@dent.tokushima-u.ac.jp  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Jun-ichi Kido, Mika Bandou, Yuka Hiroshima, Hiroyuki Iwasaka, Keisuke Yamada, Naoto Ohgami, Toshiyuki Namubu, Masatoshi Kataoka, Takenori Yamamoto, Yasuo Shinohara, Ikuko Sagawa and Toshihiko Nagata : Analysis of proteins in human gingival crevicular fluid by mass spectrometry, Journal of Periodontal Research, Vol.47, No.4, 488-499, 2012.
欧文冊子 ● Jun-ichi Kido, Mika Bandou, Yuka Hiroshima, Hiroyuki Iwasaka, Keisuke Yamada, Naoto Ohgami, Toshiyuki Namubu, Masatoshi Kataoka, Takenori Yamamoto, Yasuo Shinohara, Ikuko Sagawa and Toshihiko Nagata : Analysis of proteins in human gingival crevicular fluid by mass spectrometry, Journal of Periodontal Research, Vol.47, No.4, 488-499, 2012.

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