| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 大島 敏久 ([九州大学])
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| 2. | 川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
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| 3. | (英) Kanai Yuichiro (日) (読)
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| 4. | 郷田 秀一郎 ([長崎大学])
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| 5. | 櫻庭 春彦
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| ○題名 (必須): | □ | (英) Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii. (日)
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| ○要約 (任意): | □ | (英) 2-Keto-3-deoxygluconate kinase (KDGK) catalyzes the ATP-dependent phosphorylation of 2-keto-3-deoxygluconate, a key intermediate in the modified (semi-phosphorylative) Entner-Doudoroff (ED) glucose metabolic pathway. We identified the gene (ORF ID: ST2478) encoding KDGK in the hyperthermophilic archaeon Sulfolobus tokodaii based on the structure of a gene cluster in a genomic database and functionally expressed it in Escherichia coli. The expressed protein was purified from crude extract by heat treatment and two conventional column chromatography steps, and the partial amino acid sequence in the N-terminal region of the purified enzyme (MAKLIT) was identical to that obtained from the gene sequence. The purified enzyme was extremely thermostable and retained full activity after heating at 80 degrees C for 1 h. The enzyme utilized ATP or GTP, but not ADP or AMP, as a phosphoryl donor and 2-keto-3-deoxy-D-gluconate or 2-keto-D-gluconate as a phosphoryl acceptor. Divalent cations including Mg(2+), Co(2+), Ni(2+), Zn(2+) or Mn(2+) were required for activity, and the apparent Km values for KDG and ATP at 50 degrees C were 0.027 mM and 0.057 mM, respectively. The presence of KDGK means that the hyperthermophilic archaeon S. tokodaii metabolizes glucose via both modified (semi-phosphorylative) and non-phosphorylative ED pathways. (日)
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| ○キーワード (推奨): | 1. | (英) Archaeal Proteins (日) (読)
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| 2. | (英) Cations, Divalent (日) (読)
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| 3. | (英) Cloning, Molecular (日) (読)
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| 4. | 大腸菌 (Escherichia coli)
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| 5. | グルコース (glucose)
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| 6. | (英) Hydrogen-Ion Concentration (日) (読)
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| 7. | (英) Metals (日) (読)
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| 8. | リン酸化 (phosphorylation)
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| 9. | (英) Phosphotransferases (Alcohol Group Acceptor) (日) (読)
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| 10. | (英) Purine Nucleotides (日) (読)
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| 11. | (英) Sulfolobus (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Protein Expression and Purification ([Elsevier])
(pISSN: 1046-5928, eISSN: 1096-0279)
| ○ISSN (任意): | □ | 1046-5928
ISSN: 1046-5928
(pISSN: 1046-5928, eISSN: 1096-0279) Title: Protein expression and purificationTitle(ISO): Protein Expr PurifPublisher: Academic Press Inc. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 54
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 73 78
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| ○年月日 (必須): | □ | 西暦 2007年 2月 28日 (平成 19年 2月 28日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.pep.2007.02.013 (→Scopusで検索)
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| ○PMID (任意): | □ | 17407821 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.Affiliation: Microbial Genetics Division, Institute of Genetic Resources, Kyushu University, 6-10-1 Hakozaki Higashi-ku, Fukuoka 812-8581, Japan. ohshima@agr.kyushu-u.ac.jp (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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