『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Fukui Naoshi (日) (読)
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[継承]
2. (英) Ikeda Yasuko (日) (読)
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[継承]
3. (英) Tanaka Nobuho (日) (読)
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[継承]
4. (英) Wake Masahiro (日) (読)
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[継承]
5.山口 鉄生 ([徳島大学.大学院社会産業理工学研究部.社会総合科学域.人間科学系.スポーツ健康分野]/[徳島大学.総合科学部.社会総合科学科.心身健康コース])
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[継承]
6. (英) Mitomi Hiroyuki (日) (読)
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[継承]
7. (英) Ishida Satoru (日) (読)
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[継承]
8. (英) Furukawa Hiroshi (日) (読)
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[継承]
9. (英) Hamada Yoshiki (日) (読)
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[継承]
10. (英) Miyamoto Yoshinari (日) (読)
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[継承]
11. (英) Sawabe Motoji (日) (読)
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[継承]
12. (英) Tashiro Toshiyuki (日) (読)
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13. (英) Katsuragawa Yozo (日) (読)
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14. (英) Tohma Shigeto (日) (読)
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[継承]
題名 (必須): (英) αvβ5 integrin promotes dedifferentiation of monolayer-cultured articular chondrocytes.  (日)    [継承]
副題 (任意):
要約 (任意): (英) When cultured in monolayers, articular chondrocytes undergo an obvious phenotypic change. Although the involvement of integrins has been suggested, the exact mechanisms of the change have not been determined. This study was undertaken to clarify the mechanisms underlying the loss of chondrocyte phenotype early after plating. Primary cultured human articular chondrocytes were used for the experiments. Involvement of respective integrins in the phenotypic change was investigated in RNA interference (RNAi) experiments. A signaling pathway involved in the change was identified in experiments using specific inhibitors and adenoviruses encoding mutated genes involved in the pathway. Adenoviruses carrying mutated GTPases were used to determine the involvement of small GTPases in the process. In monolayer-cultured chondrocytes, suppression of αv or β5 integrin expression by RNAi inhibited morphologic changes in the cells and increased (or prevented a reduction in) the expression of various cartilage matrix genes. Consistent results were obtained in experiments using a blocking antibody and a synthetic inhibitor of αvβ5 integrin. The decrease in cartilage matrix gene expression in chondrocytes after plating was mediated by ERK signaling, which was promoted primarily by αvβ5 integrin. In articular chondrocytes, the affinity of αvβ5 integrin for ligands was regulated by the small GTPase R-Ras. R-Ras was gradually activated in monolayer-cultured chondrocytes after plating, which caused a gradual decline in cartilage matrix gene expression through enhanced αvβ5 integrin activation and the subsequent increase in ERK signaling. Our findings indicate that αvβ5 integrin may be involved in the change that occurs in monolayer-cultured chondrocytes after plating.  (日)    [継承]
キーワード (推奨): 1.分散分析 (analysis of variance) [継承]
2. (英) Blotting, Western (日) (読) [継承]
3. (英) Cartilage, Articular (日) (読) [継承]
4. (英) Cell Dedifferentiation (日) (読) [継承]
5. (英) Cells, Cultured (日) (読) [継承]
6. (英) Chondrocytes (日) (読) [継承]
7. (英) Humans (日) (読) [継承]
8.免疫組織化学 (immunohistochemistry) [継承]
9. (英) RNA Interference (日) (読) [継承]
10. (英) Receptors, Vitronectin (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Arthritis and Rheumatism (American Rheumatism Association/Arthritis Foundation/American Rheumatism Association/American College of Rheumatology)
(pISSN: 0004-3591, eISSN: 1529-0131)

ISSN (任意): 1529-0131
ISSN: 0004-3591 (pISSN: 0004-3591, eISSN: 1529-0131)
Title: Arthritis and rheumatism
Title(ISO): Arthritis Rheum
Publisher: John Wiley & Sons Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
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(必須): 63 [継承]
(必須): 7 [継承]
(必須): 1938 1949 [継承]
都市 (任意):
年月日 (必須): 西暦 2011年 7月 初日 (平成 23年 7月 初日) [継承]
URL (任意):
DOI (任意): 10.1002/art.30351    (→Scopusで検索) [継承]
PMID (任意): 21425129    (→Scopusで検索) [継承]
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WOS (任意): 000292809700022 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1002/art.30351  (日)    [継承]
2.(英) Article.Affiliation: National Hospital Organization Sagamihara Hospital, Sagamihara, Japan. n-fukui@sagamihara-hosp.gr.jp  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Naoshi Fukui, Yasuko Ikeda, Nobuho Tanaka, Masahiro Wake, Tetsuo Yamaguchi, Hiroyuki Mitomi, Satoru Ishida, Hiroshi Furukawa, Yoshiki Hamada, Yoshinari Miyamoto, Motoji Sawabe, Toshiyuki Tashiro, Yozo Katsuragawa and Shigeto Tohma : αvβ5 integrin promotes dedifferentiation of monolayer-cultured articular chondrocytes., Arthritis and Rheumatism, Vol.63, No.7, 1938-1949, 2011.
欧文冊子 ● Naoshi Fukui, Yasuko Ikeda, Nobuho Tanaka, Masahiro Wake, Tetsuo Yamaguchi, Hiroyuki Mitomi, Satoru Ishida, Hiroshi Furukawa, Yoshiki Hamada, Yoshinari Miyamoto, Motoji Sawabe, Toshiyuki Tashiro, Yozo Katsuragawa and Shigeto Tohma : αvβ5 integrin promotes dedifferentiation of monolayer-cultured articular chondrocytes., Arthritis and Rheumatism, Vol.63, No.7, 1938-1949, 2011.

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