| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 溝渕 佳史
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| 2. | 松﨑 和仁
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| 3. | 桑山 一行
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| 4. | (英) Kitazato K (日) 北里 慶子 (読) きたざと けいこ
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| 5. | 牟礼 英生
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| 6. | 影治 照喜
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| 7. | 永廣 信治
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| ○題名 (必須): | □ | (英) REIC/Dkk-3 induces cell death in human malignant glioma (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The progression of glioma to more malignant phenotypes results from the stepwise accumulation of genetic alterations and the consequent disruption of the apoptotic pathway and augmentation of survival signaling. REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of the growth of several human cancers; however, to date it has not been identified in brain tumors. We compared the gene and protein expression of REIC/Dkk-3 in human malignant glioma and normal brain tissues using quantitative real-time PCR, Western blotting, and immunohistochemistry. We also performed small interfering REIC/Dkk-3 (siREIC/Dkk-3) knockdown and REIC/Dkk-3 overexpression experiments to examine the role of REIC/Dkk-3 in human malignant glioma cells in vitro. In brain tissue from patients with malignant glioma, the gene and protein expression of REIC/Dkk-3 was lower than in normal brain tissue and was related to the malignancy grade. In the primary glioblastoma cell line, REIC/Dkk-3 transfection led to apoptosis owing to the activation of phosphorylated JUN, caspase-9, and caspase-3 and the reduction of beta-catenin; in REIC/Dkk-3 knockdown experiments, cell growth was augmented. Our results suggest that REIC/Dkk-3 regulates the growth and survival of these cells in a caspase-dependent and -independent way via modification of the Wnt signaling pathway. Our work is the first documentation that the gene and protein expression of REIC/Dkk-3 is down-regulated in human malignant glioma. Our demonstration of the mechanisms underlying REIC/Dkk-3-induced cell death indicates that REIC/Dkk-3 plays a pivotal role in the biology of human malignant glioma and suggests that REIC/Dkk-3 is a promising candidate for molecular target therapy. (日)
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| ○キーワード (推奨): | 1. | (英) Apoptosis (日) (読)
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| 2. | (英) Blotting, Western (日) (読)
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| 3. | (英) Brain Neoplasms (日) (読)
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| 4. | (英) Cell Line, Tumor (日) (読)
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| 5. | (英) Flow Cytometry (日) (読)
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| 6. | (英) Fluorescent Antibody Technique (日) (読)
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| 7. | (英) Gene Expression (日) (読)
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| 8. | (英) Glioma (日) (読)
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| 9. | (英) Humans (日) (読)
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| 10. | (英) Immunohistochemistry (日) (読)
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| 11. | (英) In Situ Nick-End Labeling (日) (読)
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| 12. | (英) Intercellular Signaling Peptides and Proteins (日) (読)
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| 13. | (英) Oligonucleotide Array Sequence Analysis (日) (読)
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| 14. | (英) Protein Array Analysis (日) (読)
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| 15. | (英) RNA, Small Interfering (日) (読)
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| 16. | (英) Reverse Transcriptase Polymerase Chain Reaction (日) (読)
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| 17. | (英) Signal Transduction (日) (読)
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| 18. | (英) Transfection (日) (読)
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| 19. | (英) beta Catenin (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Neuro-Oncology (Society for Neuro-Oncology)
(pISSN: 1522-8517, eISSN: 1523-5866)
| ○ISSN (任意): | □ | 1522-8517
ISSN: 1522-8517
(pISSN: 1522-8517, eISSN: 1523-5866) Title: Neuro-oncologyTitle(ISO): Neuro OncolPublisher: Oxford University Press (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 10
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| ○号 (必須): | □ | 3
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| ○頁 (必須): | □ | 244 253
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2008年 6月 初日 (平成 20年 6月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1215/15228517-2008-016 (→Scopusで検索)
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| ○PMID (任意): | □ | 18443132 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000256974900003
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1215/15228517-2008-016 (日)
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| 2. | (英) Article.Affiliation: Department of Neurosurgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, 3-18-15, Kuramoto-cho, Tokushima, Japan 770-8503. mizo@yj8.so-net.ne.jp (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) OtherID: PMC2563047 (日)
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